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水產研究(1993年創刊)

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  • 日期: 2019-05-22
水產研究(1993年創刊)-達氏深水尾魟 (Plesiobatis davies) 之完整粒線體DNA序列分析
主題: 達氏深水尾魟 (Plesiobatis davies) 之完整粒線體DNA序列分析
摘要(中):
本研究利用Long PCR及primer walking的方式將達氏深水尾魟完整粒線體DNA完成定序,並分析基因排序與組成。達氏深水尾魟完整粒線體DNA全長17514個鹼基對,共由37個基因所組成,包含2個核糖核酸基因 (rns、rnl)、13個蛋白質基因 (atp6、atp8、cox1-3、cob、nad1-6、nad4L) 及22個傳遞者核酸基因 (tRNA),與其他脊椎動物相似。達氏深水尾魟與其他7種軟骨魚類的完整粒線體基因組的排序上並無明顯不同,但是達氏深水尾魟其介於tRNAPro 和 tRNAPhe之間的主要非編碼區,又稱D-loop,是目前已知的軟骨魚序列中最長的,共 1830個鹼基對。而這段序列包含了兩段47個鹼基對的重複性片段,以及兩段具有保守性特徵的片段。建構其親緣關係樹後發現,達氏深水魟在演化上是與六鰓魟科為姊妹群,並且屬於燕魟亞目的基群。
出版日期: 2012/6/30
標題title(英): The Complete Mitochondrial DNA Sequence of the Deepwater Stingray Plesiobatis daviesi (Wallace, 1967): Unique Features in the Mitochondrial D-loop Region
作者: 蕭聖代‧徐基新‧劉燈城‧陳義雄
卷別: 20
期別: 1
頁碼: 1-16
作者 auther(英): Sheng-Tai Hsiao, Chi-Hsin Hsu, Don-Chung Liu and I-Shiung Chen
摘要abstract(英): The 17514-nucleotide mitochondrial DNA (mtDNA) sequence of the deepwater stingray (Plesiobatis daviesi) was determined using long PCR and primer walking methods. The deepwater stingray genome contains 37 genes, including 2 ribosomal RNAs, 22 transfer RNAs (tRNAs), and 13 protein-coding genes, which are similar to other vertebrates. A comparison of the deepwater stingray mtDNA sequence with that of seven completely sequenced chondrichthyan mtDNAs revealed an identical gene order. However, the major noncoding region of the deepwater stingray genome, a D-loop sequence between the tRNAPro and tRNAPhe, is the longest (1830 bp) among all known available sequences of chondrichthyan species. The components of D-loop sequences are two major copies of 47-bp tandem repeats and two characteristic conserved sequence blocks in this region. Phylogenetic analyses suggest that the deepwater stingray belongs to the basal group of Myliobatoidei that forms a sister group with Hexatrygonidae.
使用語言: 英文
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