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水產研究(1993年創刊)

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  • 點閱: 2102
  • 日期: 2019-07-16
水產研究(1993年創刊)-台灣蓄養鱟的血細胞量季節性變化
主題: 台灣蓄養鱟的血細胞量季節性變化
摘要(中):
本研究對從野外捕獲經人工馴養的三棘鱟,於2010年隨機取樣採雄鱟10隻、雌鱟10隻,每月每隻鱟重複各採集30 mL血,為重複採血組;另於2011年每月不分雌雄隨機挑選鱟20隻,但各月所採鱟檢體不得重複,每隻各採30 mL鱟血,為不重複採血組。所採鱟血加入抗凝劑後,以10倍的福馬林固定,再以人工血球計數器計算血細胞數量,分析每月鱟血細胞量的變化。重複與不重複採血組有類似的結果,血細胞量與性別、季節有關,雄鱟平均血細胞數 (20,445/µL及24,191/µL) 量較雌鱟 (17,865/µL及20,067/µL) 多,夏季與秋季的平均血細胞數 (23,334/µL及25,141/µL) 較春、冬兩季的平均血細胞數高,而其中以5至9月平均血細胞量 (25,051/µL) 為最多,可做為製作三棘鱟鱟試劑的採血適期。
出版日期: 2012/6/30
標題title(英): Seasonal Change of Hemocyte Counts of Captive Horseshoe Crab (Tachypleus tridentatus) in Taiwan
作者: 謝明昌‧蔡惠萍‧黃丁士‧張志堅‧張錦宜‧陳其欽‧黃信仁‧蔡萬生‧林金榮
卷別: 20
期別: 1
頁碼: 61-69
作者 auther(英): Ming-Chang Hsieh, Hui-Ping Tsai, Ting-Shih Huang, Chih-Chien Chang, Chin-I Chang, Chi-Kin Chang,
摘要abstract(英): The domesticated horseshoe crabs (Tachypleus tridentatus) were collected from outdoor habitats and used on this study. In 2010, 10 males and 10 females were randomly picked and each was repeatedly drawn blood 30 mL every month. In 2011, 20 horseshoe crabs were randomly picked every month. Sampled horseshoe crab was drawn blood 30 ml each and had no extra blood drowning for the rest of months. The blood samples were mixed with anticoagulant and fixed by 10 times of formalin. The hemocytes were counted by the blood cell counter and used for analysis of monthly changes. Results showed that the numbers of hemocyte were correlated with sex and seasons. The means of number of hemocyte for male horseshoe crabs (20,445/µl and 24,191/µl, sampled in 2010 and 2011, respectively) were more than for those of female (17,865/µl and 20,067/µl, sampled in 2010 and 2011, respectively). The blood samples collected in summer and autumn had more hemocytes (23,334/µl and 25,141/µl, respectively) than other seasons. The mean of hemocytes sampled from May to September (25,051/µL) was higher than rest periods of the year. Considering to have more hemocytes in blood, the applicable period to harvest horseshoe crab blood for making Tachypleus hemocytes lysate could be from May to September.
使用語言: 中文
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