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水產研究(1993年創刊)

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  • 日期: 2019-05-20
水產研究(1993年創刊)-以吸光值推估擬球藻之乾重
主題: 以吸光值推估擬球藻之乾重
摘要(中):
油產率與油脂含量是用來評估產製生質柴油藻種與養殖策略相當重要的指標,又均以藻體的乾重作為運算之基準。因為測定乾重之操作繁瑣,有些研究以容易測定的吸光值來推估取代實測乾重。本研究首先探討不同去鹽方法,對油含量高的海水擬球藻乾重的影響。結果顯示,未處理組的乾重顯著高於以逆滲透水、甲酸銨或碳酸氫銨處理組,最多差2.4倍,而以經離心及逆滲透水洗至導電度值為零組顯著最低,以其作為後續試驗之測定法。其次探討擬球藻藻液之吸光值與乾重間的關係,發現四種不同培養條件取得之藻液,可作出四條獨立的線性方程式,R2值達0.990 ~ 0.998。接著比較三種不同養殖條件下OD682大於1.0藻液之實測乾重,與由其稀釋為0.4 及0.8之二種稀釋液之實測吸光值導入線性方程式得到的估算乾重,發現實測乾重與估算乾重之誤差值高達 -20 ~ 48%。但以稀釋液實測乾重,回乘稀釋倍率之計算乾重,與原液實測乾重之誤差則較小,在 -1.63 % ~ 3.62% 之間。由此可知擬球藻的生質量,必須以逆滲透水去鹽後的實測乾重來表示,並據以計算為油脂含量與產率,吸光值則作為增殖趨勢之參考。
出版日期: 2012/12/31
標題title(英): Estimation of Biomass Dry Weight of Nannochloropsis oceanica Using Optical Density
作者: 蘇惠美‧鄭凱澤‧王淑欣‧陳紫媖
卷別: 20
期別: 2
頁碼: 49-57
作者 auther(英): Huei-Meei Su, Kai-Tse Cheng, Sui-Sin Wang and Tzyy-Ing Chen
摘要abstract(英): Lipid productivity, calculated from the biomass production and lipid content, is a key characteristic of the algal species and the culture strategy for biodiesel production. Both biomass production and lipid content are based on the biomass dry weight (DW). Due to inconvenient operation of the exactly measured DW, some reports used the estimated biomass DW by the optical density measurements and their calibration curves. At first, the different de-salted methods for DW determination of marine microalgae Nannochloropsis oceanica, a candidate for biodiesel production, were tested. The DW of non-washed samples was at least 2.4 times higher than those washed by reverse osmosis (RO) water, 0.5 M ammonium formate or 0.5 M ammonium bicarbonate or EC method (algal centrifuged and then washed with RO water till its electric conductivity became zero). The DW of EC method was the significantly lowest (p < 0.05) and used in the following experiments. The independent calibration curves from OD682 and DW were obtained by linear regression (R2 = 0.990 ~ 0.998) from 4 different culture conditions. Compared the measured DW of three condensed algal (OD682 > 1) and their deduced DW, estimated from measured OD682 of their diluted solutions and the linear equations, a great difference was found with the errors from -20% to 48%. The errors that compared the measured DW of the original algal cultures was compared with the calculated DW from the measured DW of their diluted solutions multiplied by dilution factor were lower (-1.63 ~ 3.62%). The results indicated that the biomass concentration of N. oceanica might be determined by DW measurement of de-salted algal taht washed with RO water, and then used for its lipid content or productivity calculation. The OD682 may be used as a reference of the growth of algal culture.
使用語言: 中文
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