|主題：||水產複方保護人類神經細胞 (SH-SY5Y) 及降低氧化傷害之能力|
本研究係將魷魚 (Dosidicus gigas) 皮及石蓴 (Ulva lactuca) 分別製備成魷魚皮酵素水解物和石蓴醣溶液，並以 7：3 之配比，研發具有高抗氧化能力的水產複方 (aquatic formula)。試驗目的係探討水產複方在過氧化氫 (hydrogen peroxide) 所誘導之氧化壓力下，對人類神經母細胞瘤細胞株 (neuroblastoma cells, SH-SY5Y) 的保護作用。MTT 試驗結果顯示，水產複方 (1.25、2.5及5 mg/ml) 對 SH-SY5Y 不具細胞毒性。在氧化壓力下，水產複方可藉由提高細胞內抗氧化酵素，例如超氧歧化酶 (superoxide dismutase, SOD)、觸酶 (catalase, CAT) 及穀胱甘肽過氧化酶 (glutathione peroxidase, GPx) 等活性和降低活性氧 (reactive oxygen species, ROS) 的表現量，進而達到保護細胞及降低氧化傷害的效果。綜合實驗結果顯示：水產複方因具有抗氧化和保護神經細胞的雙重能力，應可作為神經退化症的保健食品之發展潛力。
|標題title(英)：||Neuroprotective Effects of a Aquatic Formula Against Oxidative Stress-induced Damage for SH-SY5Y Human Neuroblastoma Cells|
|作者 auther(英)：||Huey-Jine Chai, Hsin-Chueh Ho and Shu-Han Yang|
|摘要abstract(英)：||A aquatic formula composed of 70% squid skin hydrolysates and 30% Ulva sugar solution (V/V) were extracted from squid (Dosidicus gigas) skins and Ulva lactuca, respectively. The current study was conducted in order to evaluate the neuroprotective effects of aquatic formula with highly antioxidant properties on hydrogen peroxide (H2O2)-induced oxidative damage. In vitro data demonstrated that there was no cytotoxic effect on human neuroblastoma cells (SH-SY5Y) when using 1.25, 2.5, and 5 mg/ml of the aquatic formula by the MTT assays. The aquatic formula in this study was also able to protect SH-SY5Y cells from H2O2-induced oxidative damage by increasing the intracellular antioxidant enzymes (including superoxide dismutase, catalase, and glutathione peroxidase) and lowering the total amount of reactive oxygen species (ROS).
The aquatic formula possessed dual antioxidant activities and neuron protective abilities, which could be capable of protecting SH-SY5Y cells against H2O2-induced oxidative damage. Thus, aquatic formula may have the potential to be developed into nutraceutics for some neurodegenerative diseases.