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水產研究(1993年創刊)

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  • 日期: 2019-05-20
水產研究(1993年創刊)-利用反應曲面法探討龍鬚菜類蕈孢素胺基酸之較適萃取條件
主題: 利用反應曲面法探討龍鬚菜類蕈孢素胺基酸之較適萃取條件
摘要(中):
類蕈孢素胺基酸 (Mycosporine-like amino acids, MAAs) 是海洋生物的胞內物質,具有吸收UV光的能力,藉以保護海洋生物避免受光傷害,然有關MAAs的萃取方式並無確切的溶劑、濃度、溫度及時間流程。在考量成本及安全性下,本研究使用乙醇作為萃取龍鬚菜Gracilaria tenuistipitata MAAs的溶劑。接續運用反應曲面法 (Response surface methodology) 探討以乙醇萃取龍鬚菜G. tenuistipitata MAAs之較適化萃取條件,在3D反應曲面圖中得知較適操作條件之交集:萃取溫度 (X1) 63℃、萃取時間 (X2) 165 min及乙醇濃度 (X3) 74.3% 為龍鬚菜MAAs的較佳萃取條件,所得粗萃物及MAAs萃取率分別為4.1 mg/g 及 0.83 mg/g 乾藻。此外,龍鬚菜MAAs粗萃物能促進HS68纖維母細胞增生及對減緩UV-B照射所造成之細胞損傷,推測:龍鬚菜MAAs粗萃物具有開發作為天然防曬劑之可行性。
出版日期: 2015/12/30
標題title(英): Optimizing Conditions for Mycosporine-like Amino Acids of Gracilaria tenuistipitata Using Response Surface Methodology
作者: 洪郁嵐‧黃培安‧朱永淳‧吳純衡
卷別: 23
期別: 2
頁碼: 75-87
作者 auther(英): Yu-Lan Hung, Pai-An Hwang, Yung-Chun Chu and Chwen-Herng Wu
摘要abstract(英): Mycosporine-like amino acids (MAAs) are a family of intracellular ultraviolet (UV) -absorbing compounds involved in the protection of aquatic organisms against solar UV radiation. The protocols used to extract MAAs largely differ; for example, differences include the type of solvent, concentrations, temperatures, and times used. Since ethanol is less expensive and less toxic than methanol, the procedure used in this study to extract the MAAs of Gracilaria tenuistipitata was based on ethanol. An optimization study of G. tenuistipitata MAA extraction using response surface methodology (RSM) was performed, and 3D response surfaces were plotted from the mathematical models. The optimal conditions based on combination responses were as follows: a temperature (X1) of 63℃, a time (X2) of 165 min, an ethanol concentration (X3) of 74.3% ethanol. Under the optimum extraction procedure conditions, the yields of crude MAAs and MAAs were 4.1 mg/g and 0.83 mg/g dry weight. Additionally, crude MAAs of G. tenuistipitata significantly increased cell proliferation and protected against UVB-induced damage in HS68 fibroblast cells. Our study revealed that crude MAAs of G. tenuistipitata present a potential source of natural photoprotective compounds.
使用語言: 中文
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