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水產研究(1993年創刊)

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  • 日期: 2019-07-19
水產研究(1993年創刊)-自海水魚蝦篩選抗水產病原弧菌之拮抗菌
主題: 自海水魚蝦篩選抗水產病原弧菌之拮抗菌
摘要(中):
水產病原弧菌所引起的弧菌疾病長久以來都是水產養殖產業的重大威脅之一,急需研發微生物防治策略以控制弧菌疾病。本研究擬自市場販售之海水魚蝦中分離篩選具有抗水產病原弧菌之拮抗菌,挑選抗弧菌能力強的菌株,進一步探討其對抗生素之耐受性,以做為將來應用之參考。本研究共購買4種海水魚蝦樣品,自其中分離純化出84個菌株,將上述菌株分別測試其對常見水產病原弧菌-溶藻弧菌 (Vibrio alginolyticus)、腸炎弧菌 (V. parahaemolyticus) 及哈維氏弧菌 (V. harveyi) 的抑制能力。經比較其抗弧菌能力後發現,較強的是來自白蝦樣品所分離出的D5菌株,D5菌株依其革蘭氏染色、相關生理、生化特性及16S rRNA基因分析鑑定為Bacillus pumilus,並命名為Bacillus pumilus D5。進一步探討Bacillus pumilus D5菌株對不同水產病原弧菌之抑菌情形顯示,該菌株除了對於溶藻弧菌、腸炎弧菌及哈維氏弧菌有抑制能力外,對於鰻弧菌 (V. anguillarum) 、霍亂弧菌 (V. cholerae) 及創傷弧菌 (V. vulnificus) 亦有抑制作用。此外,B. pumilus D5也具有抑制大腸桿菌 (Escherichia coli) 及鼠傷寒沙門氏菌 (Salmonella typhimurium) 的能力。進一步測試B. pumilus D5 對15種水產常用抗生素耐受性試驗,結果顯示,除了對於lincomycin具抵抗力外,對於其餘14種抗生素均為敏感性。以上結果顯示,B. pumilus D5具有抑制溶藻弧菌、腸炎弧菌、哈維氏弧菌、鰻弧菌、霍亂弧菌及創傷弧菌等6種常見水產病原弧菌之能力,且對於所測試14種抗生素皆呈現敏感性,具有做為抗水產病原弧菌的拮抗菌之發展潛力。
出版日期: 2016/6/30
標題title(英): Screening Bacteria Antagonistic toward Aquatic Pathogenic Vibrio spp. from Marine Fishes and Shrimp
作者: 朱惠真‧黃美瑩‧劉旭展‧曾亮瑋‧潘崇良‧張錦宜
卷別: 24
期別: 1
頁碼: 37-50
作者 auther(英): Huei-Jen Ju, Mei-Ying Huang, Hsu-Chan Liu, Liang-Wei Tseng, Chorng-Liang Pan and Chin-I Chang
摘要abstract(英): There is an urgent need to develop microbial control strategies to control vibriosis outbreaks in aquaculture industries. The present work was devoted to screening for aquatic pathogenic Vibrio spp. antagonistic bacteria in marine fishes and shrimp. The sensitivities of the probiotic to various antibiotics were tested as well. In the present study, a total of 84 isolates were isolated from four samples. All the isolates were tested against pathogenic Vibrio spp., including V. alginolyticus, V. parahaemolyticus, and V. harveyi. Among the 84 isolates, the strain D5, isolated from white shrimp, showed high antagonistic abilities toward pathogenic Vibrio spp. The strain D5 was identified as Bacillus pumilus by Gram stain and 16S rRNA sequences, as well as by its physiological and biochemical characteristics, and was thus named Bacillus pumilus D5. The B. pumilus D5 exhibited antagonism against V. alginolyticus, V. anguillarum, V. cholerae, V. harveyi, V. parahaemolyticus, and V. vulnificus. Moreover, Escherichia (E.) coli and Salmonella typhimurium were also inhibited by the B. pumilus D5. To test the sensitivity of the B. pumilus D5, 15 different antibiotics were used. The B. pumilus D5 exhibited at least some sensitivity to 14 different antibiotics tested in this study, while it was resistant to lincomycin. In conclusion, the B. pumilus D5 exhibited antagonism against V. alginolyticus, V. anguillarum, V. cholera, V. harveyi, V. parahaemolyticus, and V. vulnificus, as well as sensitivity to most of the antibiotics tested in this study. The present study results reveal that the B. pumilus D5 has potential applications for controlling pathogenic Vibrio spp. in aquaculture practices.
使用語言: 中文
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