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水產研究(1993年創刊)

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  • 日期: 2019-11-15
水產研究(1993年創刊)-誘引劑經由活化神經胜肽F基因表現以促進白蝦食慾及生長
主題: 誘引劑經由活化神經胜肽F基因表現以促進白蝦食慾及生長
摘要(中):
神經胜肽F (neuropeptide F, NPF) 是類似脊椎動物神經胜肽Y (neuropeptide Y) 的一種胜肽,主要調控無脊椎動物的攝食、代謝、繁殖和對於外來刺激的反應。攝食調控基因廣泛分佈於中樞和週邊神經系統,藉由兩者之間的相互調控而影響食慾與攝食行為。本實驗在研究攝食因子–NPF基因之表現對白蝦 (Litopenaeus vannamei) 攝食之影響,以及 NPF 在白蝦各組織中的表現量,經由 RT-PCR 分析結果顯示,NPF 基因表現在神經節和腦組織中較高。在固定攝食週期下,白蝦神經節 NPF 基因之表現,在攝食前 1 hr,NPF 基因的表現出現顯著上升,攝食後,NPF基因表現量逐漸降低。此外,大蒜萃取物、硫代甜菜鹼 (DMPT) 及氧化三甲胺 (TMAO) 三種誘引劑皆能顯著促進白蝦中 NPF 基因的表現量。且混合使用 DMPT 及 TMAO 則具有加乘作用。攝食前處理 TMAO 則可使白蝦 NPF 基因表現量顯著上升 (p < 0.05),可縮短找尋飼料的時間、增加其攝食量。本研究證實誘引劑藉由影響攝食調控基因NPF表現增進食慾與攝食,並確定 NPF 與甲殼類攝食及其行為有關,是白蝦重要的促攝食調控因子。
出版日期: 2016/12/31
標題title(英): Attractants Activate Neuropeptide F Gene Expression to Promote Appetite and Growth in White Shrimp (Litopenaeus vannamei)
作者: 宋嘉軒‧陳亭宇‧陸振岡
卷別: 24
期別: 2
頁碼: 45-55
作者 auther(英): Chia-Hsuan Sung, Ting-Yu Chen and Jenn-Kan Lu
摘要abstract(英): Neuropeptide F (NPF) is one of the homologs of vertebrate neuropeptide Y and mainly regulates food intake, metabolism, reproduction, and stress reaction in invertebrate. Feeding regulation genes are widely distributed in the central and peripheral nervous systems and influence appetite and feeding behavior via the inter-regulatory relationship between the two systems. This experiment investigated the effect of the expression of a feeding regulation gene, NPF, on Litopenaeus vannamei feeding behaviors and examined its expression in different tissues of shrimp. The results of semi-quantitative RT-PCR analysis showed that NPF gene was dominantly expressed in ganglia and brain. Under the fixed feeding periodicity, the NPF gene expression level significantly increased one hour before feeding, and gradually decreased after feeding. Such attractants as garlic extract, dimethyl propiothetin (DMPT), and trimethylamine N–oxide (TMAO) can significantly promote NPF gene expression in white shrimp. A combined use of DMPT and TMAP has a synergistic effect. TMAO utilization before feeding can significantly increase NPF gene expression in white shrimp (p < 0.05), shortening the time they need to search for forage and improving food intake. In this study, we have demonstrated that attractants can improve appetite and ingestion by effecting the expression of NPF gene and showed the relation of NPF to feeding behavior of crustaceans, which makes NPF an important feed regulation gene in white shrimp.
使用語言: 中文
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