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水產研究(1993年創刊)

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  • 日期: 2019-05-20
水產研究(1993年創刊)-飼料中添加益生菌Bacillus pumilus D5對於白蝦成長、免疫反應及抗腸炎弧菌效率之影響
主題: 飼料中添加益生菌Bacillus pumilus D5對於白蝦成長、免疫反應及抗腸炎弧菌效率之影響
摘要(中):
弧菌症長久以來都是水產養殖產業的重大威脅之一,應用微生物防治策略有助於弧菌症之控制。本研究添加對弧菌具拮抗作用的益生菌Bacillus pumilus D5 於飼料中 (107 CFU/g) 餵食白蝦 (Litopenaeus vannamei) 8 週。結果顯示,白蝦增重率及飼料效率皆無顯著增加 (p > 0.05),但是試驗組在第10天,白蝦缸內的水與肝胰腺之弧菌數 (5.7 × 102 CFU/ml,7.3 × 104 CFU/g) 均明顯低於對照組 (2.6 × 103 CFU/ml,3.8 × 105 CFU/g) (p < 0.05)。免疫指標方面,試驗組第 8 週,白蝦血液中酚氧化酶 (phenoloxidase) 活性 (0.315) 及呼吸爆 (0.566) 均明顯高於對照組 (0.111,0.380) (p < 0.05),以腸炎弧菌 (Vibrio parahaemolyticus) 攻擊後,試驗組血液中酚氧化酶活性 (0.326) 亦顯著高於對照組 (0.257) (p < 0.05)。免疫相關基因方面,白蝦飼餵試驗組飼料後,以腸炎弧菌攻擊前後,唐氏綜合症細胞黏附分子 (Down syndrome cell adhesion molecule) 及原酚氧化酶 (prophenoloxidase) 基因之相對表現量 (26.72,4.83) 均明顯高於對照組 (5.46,1.60) (p < 0.05)。以腸炎弧菌攻擊後,試驗組的活存率 (16.08%) 明顯高於對照組 (4.76%) (p < 0.05)。以上結果顯示,B. pumilus D5具有降低白蝦之缸內水與肝胰腺之弧菌數,提升白蝦之免疫反應及抗腸炎弧菌感染之功能。
出版日期: 2016/12/31
標題title(英): Effects of Probiotic Bacillus pumilus D5 In-feed on Growth Performance, Immune Response, and Efficiency of Resistance to Vibrio parahaemolyticus in White Shrimp (Litopenaeus vannamei)
作者: 黃美瑩‧朱惠真‧陳力豪‧劉旭展‧曾亮瑋‧潘崇良‧張錦宜
卷別: 24
期別: 2
頁碼: 57-69
作者 auther(英): Mei-Ying Huang, Huei-Jen Ju, Li-Hao Chen, Hsu-Chan Liu, Liang-Wei Tseng, Chorng-Liang Pan and Chin-I Chang
摘要abstract(英): The use of microbial control strategies helps to control vibriosis, a disease that has long imposed a major threat to aquaculture industries. In this study, white shrimp (Litopenaeus vannamei) were fed a diet supplemented with Vibrio-antagonistic Bacillus pumilus D5 at concentration of 107 CFU/g for 8 weeks. No significant improvements of percent weight gain and feed efficiency were observed (p > 0.05). However, the numbers of Vibrio spp. in the tank water and hepatopancreas of the shrimp at day 10 were significantly lower in the experimental group (5.7 × 102 CFU/mL, 7.3 × 104 CFU/g) than in the control group (2.6 × 103 CFU/mL, 3.8 × 105 CFU/g) (p < 0.05). With regard to the immunity indicators, at week 8, the phenoloxidase activity and respiratory burst of white shrimp fed with the experimental diet (0.315, 0.566) were significantly higher than those of shrimp fed with the control diet (0.111, 0.380) (p < 0.05). The phenoloxidase activity of white shrimp after challenge with Vibrio parahaemolyticus was significantly higher in the experimental group (0.326) than in the control group (0.257) (p < 0.05). With regard to immune-related genes, the relative gene expression of Down syndrome cell adhesion molecule and prophenoloxidase before and after challenge with V. parahaemolyticus was significantly higher in white shrimp fed with the experimental diet (26.72, 4.83) than that in shrimp fed with the control diet (5.46, 1.60) (p < 0.05). The survival rate of white shrimp after challenge with V. parahaemolyticus was significantly higher in the experimental group (16.08%) than in the control group (4.76%) (p < 0.05). Overall, the results of this study indicated that B. pumilus D5 can decrease the numbers of Vibrio spp. in the tank water and hepatopancreas of white shrimp and enhance immune response and resistance to vibriosis infection in white shrimp.
使用語言: 中文
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