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水產研究(1993年創刊)

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  • 點閱: 2597
  • 日期: 2019-10-15
水產研究(1993年創刊)-尼羅吳郭魚性別決定之研究
主題: 尼羅吳郭魚性別決定之研究
摘要(中):
本研究應用吳郭魚遺傳連鎖圖譜 (linkage map),以微衛星分析兩個品系共五對尼羅吳郭魚及其子代之性別決定相關之連鎖族群 (linkage group, LG),試驗一共篩選出六個微衛星基因座 (UNH995、GM258、UNH168、GM354、UNH216及UNH898,分別位於LG1、LG3和LG23) 進行基因型分析。結果顯示,三組配對之尼羅吳郭魚 (N1-1、N1-2與N2-2家系)的性別決定與LG23等二個基因座標誌 (UNH216及UNH898) 有相關性 ( p < 0.05),顯示尼羅吳郭魚的性別決定受到LG23所影響。根據試驗結果,可推測N1-1與N1-2家系的性別決定系統為XX-XY。進一步分析N1子代在LG23的基因型,發現UNH216之對偶基因型D和UNH898之對偶基因型E在雄性之決定有關聯性。因此,本研究初步確認雄性決定之遺傳標誌,未來可應用於全雄性尼羅吳郭魚之遺傳育種與改良工作。
出版日期: 2017/6/30
標題title(英): Sex Determination of the Nile Tilapia, Oreochromis niloticus
作者: 張格銓‧陳榮華‧黃瀛生‧張芸溶‧劉富光
卷別: 25
期別: 1
頁碼: 45-53
作者 auther(英): Ke-Chuan Chang, Rong-Hwa Chen, Ying-Sheng Huang, Yun-Jung Chang and Fu-Guang Liu
摘要abstract(英): In this study, we applied genetic linkage mapping to analyze linkage groups (LG) related to sex determination in five Nile tilapia (Oreochromis niloticus) families and their offspring. Six microsatellite loci (UNH995 and GM258 on LG1; UNH168 and GM354 on LG3; UNH216 and UNH898 on LG23) were used for genotyping. The results showed that two microsatellite loci on LG23 (UNH216 and UNH898) were associated with sex determination (p<0.05) in three Nile tilapia (N1-1, N1-2, and N2-2 family), indicating that the sex determination in Nile tilapia might be influenced by LG23. Furthermore, the empirical results showed that the sex determination system in the N1-1 and N1-2 families was XX-XY. Analysis of the N1 offspring for LG23 indicated that allele D on UNH216 and allele E on UNH898 were associated with male determination. Thus, this study preliminarily confirmed that the genetic markers associated with male determination could be useful for genetic selection and improvement of genetic male Nile tilapia.
使用語言: 中文
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