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水產研究(1993年創刊)

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  • 點閱: 3643
  • 日期: 2019-10-14
水產研究(1993年創刊)-益生菌藤黃微球菌之特性測定及菌種鑑定
主題: 益生菌藤黃微球菌之特性測定及菌種鑑定
摘要(中):
由養殖池分離之菌株LK1012W進行各項形態、生理及生化分析測試,試驗結果顯示本細菌屬革蘭氏陽性球菌、觸酶反應陽性、不具氧化酶、不會產生內孢子、會生長在好氧的培養基、厭氧環境下不生長。形態、生理及生化特性試驗綜合結果顯示,LK1012W與Micrococcus luteus及M. yunnamensis最接近;進行16S rDNA部份序列分析,結果與M. luteus、M. yunnamensis、M. endophyticus、M. antarctisus等4株微球菌相似度均達98%。分析LK1012W之DNA G+C content為72.8 mol%,顯示其分類可能落在M. luteus、M. yunnamensis、M. endophyticus等3株菌種。進行DNA hybridization,結果顯示LK1012W與M. luteus BCRC 11034相似性為89%,與M. yunnanensis BCRC 80243相似性為80%。綜合以上的試驗結果,本研究將LK1012W鑑定為藤黃微球菌M. luteus。
出版日期: 2017/6/30
標題title(英): Characterization and Identification of a Probiotic Bacterium, Micrococcus luteus
作者: 黃世鈴‧黃麗玲‧楊豐隆‧冉繁華‧陳秀男
卷別: 25
期別: 1
頁碼: 55-63
作者 auther(英): Shih-Ling Huang, Li-Ling Huang, Fong-Long Yang, Fan-Hua Nan and Shiu-Nan Chen
摘要abstract(英): Morphological, physiological and biochemical characterizations were conducted for the identification of an isolated bacterium LK1012W. The results showed that the characters of LK1012W were Gram positive (+) coccus, Catalase (+), Oxidase (‒), endospore (‒), able to grow up on aerobic medium (+) and unable to grow on anaerobic medium (‒), with DNA G + C content (16S rDNA) of 72.8 mol%.
The LK1012W strain was similar to three species of Micrococcus: M. luteus,M. yunnanensis and M. endophyticus, based on 138 characteristics tested and 16s rDNA sequenced. The results of DNA hybridization showed that LK1012W vs M. luteus BCRC 11034 exhibited 89% similarity while that of LK1012W vs M. yunanensis BCRC80243 was 80%. Upon the above results, the LK1012W strain was therefore identified as M. luteus.
使用語言: 中文
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