行政院農業委員會-水產試驗所全球資訊網

  • Font Size:S
  • Font Size:M
  • Font Size:L
  • 藍
  • 綠
  • 橙
  • 紫
  • FaceBook
  • 農業創新育成中心
  • YouTube
  • Flickr
  • 觀賞魚網站
  • 水產陳列館
  • 水產食譜

水產研究(1993年創刊)

  • 書籤: facebook  plurk  funp  twitter 
  • 點閱: 1329
  • 日期: 2019-05-24
水產研究(1993年創刊)-美洲大嘴鱸腸道中葡聚糖產生菌 (Leuconostoc mesenteroides B4) 之篩選
主題: 美洲大嘴鱸腸道中葡聚糖產生菌 (Leuconostoc mesenteroides B4) 之篩選
摘要(中):
本研究自魚隻腸道篩選具有產生葡聚糖能力之細菌,估算其葡聚糖產生量、分析其所產葡聚糖之結構特性並鑑定該葡聚糖產生細菌之物種。結果顯示,自美洲大嘴鱸魚 (Micropterus salmoides) 腸道中篩選出1株具有合成葡聚糖能力之細菌 (B4),B4菌株於含有20% 蔗糖之液體培養基培養 48 hr (28°C),所產葡聚糖量估算為50 mg/ml。B4菌株所產葡聚糖之分子量為9.5 × 105 Da。該葡聚糖以酸液熱水解處理後,發現其單糖組成份中以葡萄糖為主。分析其1H NMR及13C NMR圖譜,顯示與文獻上其他論文報告中所述α(1-6) 葡聚糖的鍵結資料相當接近,因此推斷該B4菌株所產葡萄糖聚合物為α(1-6) 直鏈鍵結型態的葡聚糖,且有α(1-3) 支鏈。葡聚糖產生菌B4 菌株部分16S rRNA基因分析鑑定為Leuconostoc mesenteroides,並將B4 菌株命名為 L. mesenteroides B4。
出版日期: 2017/12/31
標題title(英): Screening of the Dextran-producing Bacterium, Leuconostoc mesenteroides B4, in the Intestines of Largemouth Bass (Micropterus salmoides)
作者: 黃美瑩‧朱惠真‧曾亮瑋‧許晉榮
卷別: 25
期別: 2
頁碼: 23-33
作者 auther(英): Mei-Ying Huang, Huei-Jen Ju, Liang-Wei Tseng and Jinn-Rong Hseu
摘要abstract(英): This study was devoted to the screening of a dextran-producing bacterium in the intestines of fishes. The structure of the dextran produced by the bacterium was characterized, the content of the dextran was estimated, and the dextran-producing bacterium was identified. The results indicated the presence of one strain, B4, which was isolated from largemouth bass (Micropterus salmoides) and had a dextran-producing property. The strain B4 was cultured in a 20%-sucrose nutrient medium. The amount of dextran produced by the strain B4 was 50 mg/ml after 48 hr of incubation at 28°C. The molecular weight of the dextran was 9.5 × 105 Da. The hydrolysate derived from acid and heating treatments of the dextran was mainly glucose. In comparison with previous reports, the 1H NMR and 13C NMR spectra of the dextran showed that its structure was similar to those of other dextrans which containα(1-6)-linked backbones. Thus, the glucose polymer produced by the strain B4 was identified as dextran with α(1-6)-linked backbones that hadα(1-3) branches. The strain B4 was identified as Leuconostoc mesenteroides by partial 16S rRNA gene sequencing and named L. mesenteroides B4.
使用語言: 中文
相關檔案下載 (ODF或PDF): 下載