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水產研究(1993年創刊)

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  • 日期: 2019-07-16
水產研究(1993年創刊)-魷魚皮磷脂質微脂體對LPS誘發微膠細胞發炎反應之影響
主題: 魷魚皮磷脂質微脂體對LPS誘發微膠細胞發炎反應之影響
摘要(中):
微膠細胞 (microglia cell) 為在腦部的一種巨噬細胞,在發炎反應過度活化時,會導致腦部神經元損傷。有研究顯示,巨噬細胞吞噬細胞凋亡體 (apoptotic body) 後會啟動抗發炎作用。本研究以魷魚皮磷脂質製備之微脂體來模擬細胞凋亡體,並探討以脂多醣 (lipopolyscharride, LPS) 誘導老鼠腦部微膠細胞株 (BV-2) 產生發炎反應下,是否具抗發炎之能力。以HPLC分析魷魚皮磷脂質組成分,磷脂醯膽鹼 (phosphatidylcholine, PC)、磷脂醯乙醇胺 (phosphatidylethanolamine, PE)、磷脂醯絲胺酸(phosphatidylserine, PS)、磷脂醯肌醇 (phosphatidylinositol, PI)、溶血磷脂膽鹼 (lysophosphatidylcholine, Lyso-PC) 及其他磷脂質,分別佔46.2、18.4、7.7、3.5、4.9、19.3%,磷脂質中n-3系列二十碳五烯酸 (eicosapentaenoic acid, EPA) 及二十二碳六烯酸 (docosahexaenoic acid, DHA) 分別為11.8及28.7%,製成之魷魚皮微脂體 (SQ-liposome) 大小約為100 nm。細胞存活率的結果發現濃度2.5 mg/ml以下的SQ-liposome不會影響BV-2的細胞存活率,並且於顯微鏡下可觀察到BV-2細胞會吞噬SQ-liposome。在分析SQ-liposome抑制LPS所誘導的促發炎細胞激素的結果,發現0.83與2.5 mg/ml 的SQ-liposome可顯著抑制BV-2分泌介白素-6 (interleukin-6, IL-6)、腫瘤壞死因子 (tumor necrosis factor alpha, TNF-) (p<0.05);同時也發現SQ-liposome會誘發抗發炎細胞激素,轉化生長因子 (transforming growth factor beta, TGF-)、介白素-10 (interleukin-10, IL-10) 的分泌 (p < 0.05)。另外 LPS所誘導的一氧化氮 (nitric oxide, NO) 生成,亦隨預處理SQ liposome後隨濃度增加而顯著性下降 (p < 0.05)。由上述結果顯示,SQ-liposome具有抑制LPS誘發微膠細胞發炎反應之功效,未來可作為抗腦部發炎相關疾病之應用。
出版日期: 2017/12/31
標題title(英): The Effects of Phospholipid Liposome Prepared from Squid Skin on LPS Induced Microglial Cell Inflammation
作者: 高翊峰‧林怡伶‧李偉毅‧陳億乘
卷別: 25
期別: 2
頁碼: 69-82
作者 auther(英): Yi-Feng Kao, Yi-Ling Lin, Wei-Yi Li and Yi-Chen Chen
摘要abstract(英): Microglial cell is a macrophage that resides in brain. However, overactive microglial cells may result in brain neuron damage or inflammation. Recent studies show that the initiation of anti-inflammatory is triggered by macrophage engulfed apoptotic bodies. In this study, the liposome (SQ-liposome) was made from phospholipids extracted from squid skin, and used to mimic apoptotic body. The anti-inflammatory effects of SQ-liposome were then evaluated on the lipopolysaccharide (LPS) induced mouse microglial cell line (BV-2). The major phospholipid constituents in the squid skin extract was including 46.2% of phosphatidylcholine, 18.4% of phosphatidylethanolamine, 7.7% of phosphatidylserine, 3.5% of phosphatidylinositol, 4.9% of Lysophosphatidylcholine, and 19.3% of other phospholipids in HPLC-UV analysis. An approximately 100 nm of SQ-liposome was prepared by ultrasonication. There was no cytotoxicity to BV-2 as the concentration of SQ-liposome was less than 2.5 mg/ml after microglial cells engulfed SQ-liposome. The LPS induced pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6), were significantly suppressed (p < 0.05) by pretreated 0.83~2.5mg/ml SQ liposome. Oppositely, the anti-inflammatory cytokines transforming growth factor-beta (TGF-β) and interleukin-10 (IL-10) secretion were enhanced (p < 0.05). The results suggested that SQ-liposome possess anti-inflammatory properties on BV-2 and may be a good strategy for against neuro-inflammatory disease.
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