More than 150 freshwater fish species have been identified in Taiwan. One fifth of them are endemic species or subspecies. Unfortunately ,the populations of many species have been declined because of human causes and environmental changes. Moreover, the exotic species have invased in the freshwater areas and have threaten the native population. This project is accordingly conducted to keep and breed those indigenous fish species as well as to develop the farming protocol. This year, we continue to establish the breeding methods of bitterling fish, Taiwan chub(Opsariichthys pachycephalus) , stone morocco(Pseudorasbora parva) and Taiwan torrent carp (Acrossocheilus paradoxus). In addition, it is also confirmed that mature Taiwan chub can be induced spawning repeatedly during the breeding period and the fish can spontaneous spawning without artificial stripped after injection . This study will continue to explore the methods of fish brood breeding, fertilized eggs hatching and nursery feeding , to establish a repeatability model for fish fry breeding.