1.This study is aimed to understand the reproductive behavior, larval culture of Neoglyphidodon melas under artificial environment of Neoglyphidodon melas and other relevant materials. Besides, we hope the result of the study can reduce the trade of wild fish in the ornamental fish market in the future. The results showed these adhesive demersal eggs are white to yellow and ellipsoidal, and were about 1.58 ±0.06 mm(Mean ±SD) in total length and 0.71 ±0.02 mm in width. The yolks were about 1.06 ±0.07 mm long and contained several oil droplets with diameters ranging from 0.28 ±0.02 mm and the newly hatched larvae were about 2.86 ±0.12 mm in length. Parental care behaviors for the ovum occurred after spawning to hatching and it indicates that parental behavior is mainly carried out by males. Incubation period was approximately 106 h at the water temperature of 28 ±1 ℃. The biological feedings for Blue Devil at initial larvae stage was ciliate, rotifer and copepod. Microalgae was added to stabilize water quality and to nourish rotifers. The supply of feedings should be applied at the same time. Under heat stress experiments, blue devil was caused to die at 37.2 ℃, and all fish die when the temperature reached to 38.5 ℃. In the low temperature tolerance test, fish cause to die at 15.1 ℃ and all fish die at 13.2 ℃.
2.This study aimed to artificial propagation of Peppermint Shrimp( Lysmata boggessi Rhyne &Lin, 2006). Larva feeding with the nauplii of Artemia spp. at 0.5-4/ml had better survival rate and growth on 6 dph (days post hatch). The survival rate and growth were better in which salinity at 24-39psu. Survival rate and growth decreased as larva were not fed on instantly hatched. Batter survival rate was obtained in which were raised during the 18, 21, 24 and 27℃, but better growth was obtained at 27,30 and 33℃. Overall, better survival rate and growth would be obtained under raising at 27℃, 34psu, and feeding with the nauplii of Artemia nauplii at 2- 4/ml. Larva were developing to postlarva at 36-104dph.