The objective this year aims to cross-breed the different breeding lines of the shrimp gene bank and to evaluate the heterosis and economic hybridization combination. Three white shrimp germplasm (S101, K103, and CP106) were introduced and with inbreeding and growth trait selective breeding for 1 to 4 generations. The 3 × 3 diallel analysis was conducted to test the combining ability and heterosis. The general combined ability of yield trait was a positive value when S101-F4 as female and S101-F4, CP106-F1 as male broodstock. In the hybrid combination, K103♀×CP106♂, CP106♀×K103♂, and S101♀×K103♂ have a positive special combined ability on the yield trait. All hybrid combinations showed heterosis rate, among which K103♀×CP106 ♂hybrid offspring was higher in growth (g/w), yield (kg/m2 ) and survival rate (%) than commercial line k106-F1 with 6.9%, 16.7%, and 7.5%, respectively. This hybrid line can be used as a basis for selection of parents in economic hybrid production, and can also be preserved as good varieties. The future work in addition to maintaining the original inbreeding purification and growth selection, the six hybrid combinations produced this year can also be combined to create more excellent hybrid lines.