1.Four of the fivePenaeus monodoncollected along the coast of Taiwan were detected without WSSV、TSV and IHHNV, and the larvae from the three shrimp spawning naturally was detected to be SPF one (F 1 ).
2.One hundred and three pairs of tiger shrimp from Madagascar (F 1 ) were screened for egg spawning after artificial eyestalk ablation, and two shrimp larvae of those were found to be SPF one (F 2 ).
3.One batch of tiger shrimp collected along the coast of Taiwan (F 1 ) and 2 batch of larvae of Madagascar tiger shrimp, screened with environmental impact and virus challenge, were used for cultivating and breeding at the stocking density of 50-75 individual/m2 .