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微藻營養成分分析及建置標準化保種技術

  • 日期:107-02-20
  • 計畫編號:107農科-9.3.2-水-A1(6)
  • 年度:2018
  • 領域:漁業科技研發
  • 主持人:周芷儀
  • 研究人員:黃維能、王淑欣、李念宜

本研究微藻保種部分供試藻株有1株血腥紅球藻( Porphyridium cruentum )、2株淡 水綠球藻 ( Chlorella sorokiniana , Chlorella vulgaris  )、1株螺旋藻(  Arthrospira maxima )和1株杜莎藻 ( Dunaliella salina ),共5株微藻種原。 探 討培養液之配方、鹽度、溫度與光照度對保種之影響,比較施打空氣與二氧化碳對 種原增殖之影響,建立最佳保種條件。 結果顯示最適保種光照度及溫度杜莎藻為 100umol/m2 /s及20℃、螺旋藻為50umol/m2 /s及25℃, Chlorella sorokiniana , Chlorella vulgaris 及血腥紅球藻之最適保種光照度為50umol/m2 /s及30℃;微藻擴 增培養試驗,添加微量二氧化碳及打氣培養皆可促進藻株成長。 本研究微藻應用部分供試藻株有1株血腥紅球藻( Porphyridium cruentum )、2株淡 水綠球藻 ( Chlorella sorokiniana , Chlorella vulgaris )、1株螺旋藻( Arthrospira maxima )和1株聚球藻 ( Synechococcus sp. ),共5株微藻種原,分 析各微藻所含營養成分,包含水分、灰分、粗脂質及粗蛋白含量,結果顯示聚球藻 含有較高的蛋白質,螺旋藻水分含量較高,血腥紅球藻灰分含量較高,淡水小球藻 脂質含量較高。

研究報告摘要(英)


This study aims to find optimum stock condition of microalga on strains preservation. In this study, the microalgae strains of test include Porphyridium cruentum ,Chlorella sorokiniana , Chlorella vulgaris ,  Arthrospira maxima and Dunaliella salina, a total of 5 strains of microalgae.

The effect results of the culture medium formulation, salinity, temperature and photosynthetically active radiation showed that optimum temperature and photosynthetically active radiation of microalga stock condition are 100umol/m2 /s and 20°C for culturing Dunaliella salina, 50umol/m2 /s and 25°C for culturing Arthrospira maxima, and the optimal stock condition of Chlorella sorokiniana, Chlorella vulgaris and Porphyridium cruentum was 50umol/m2 /s and 30 °C; Microalgae culture experiments, pumping trace amounts of carbon dioxide or air in can promote the growth rate of microalgae. In this study, microalgae strains of tests included Porphyridium cruentum , Chlorella sorokiniana, Chlorella vulgaris, Arthrospira maxima and Synechococcus sp., a total of 5 microalgae strains, analysis of the nutrients contained in each microalgae, the results show that Synechococcus sp. contains higher protein, Arthrospira maxima  content higher moisture, the ash content of Porphyridium cruentum is higher, and the lipid content of Chlorella sorokiniana and Chlorella vulgaris is higher.