In this study, genetic markers were applied to research YY tilapia. A group of fries mated 5 female Nile tilapia with a YY male. Besides, a marker on LG23 (FWLK03) were selected for genotyping. We tracked the offsprings genotyping and designed the growth experiment in this year. The result show the weight of offsprings Y1 and P1 have no significant difference. Therefore, 2 group (Y1+genetic female and P1+genetic female) have better growth than the others before experiment but have no significant difference when the experiment done.