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白蝦種原保存與選拔育種

  • 日期:106-03-24
  • 計畫編號:106農科-10.3.3-水-A1(4)
  • 年度:2017
  • 領域:漁業科技研發
  • 主持人:楊明樺
  • 研究人員:楊生山、吳秉儒

本計畫之目的在建立SPF白蝦種蝦庫並選育優良白蝦品系。本年度所得結果如下 :1.白蝦種原之引進:目前已引進並保存SPF白蝦種原5批。2.病原追蹤檢測:持續 以qPCR檢測技術篩選不帶WSSV、TSV、YHV/GAV、IMNV及IHHNV等6種病毒、EMS的病原 菌-副溶血弧菌及EHP的白蝦種原。3.高生殖力之選育:本試驗共使用130隻母蝦,在 為期231天的記錄中,個別產卵次數的差異很大,其中有95隻產卵次數是0,而產卵 數11次以上者共計21隻,佔130隻母蝦的16%,共產卵298次,佔總產卵741次的40%。 4.高成長之選育:本年度成長選育以集團選擇法進行三階段之高成長選育。第一階 段選擇差0.83-1.23g,選擇壓力22-34%。第二階段公蝦選擇差1.1-2.1g,選擇壓力 25-30%;母蝦選擇差1.6-3.0g,選擇壓力23-29%。5.選育效率:S101高產次有較佳的 選擇效應與現實遺傳力,分別為7.13%與0.24。6.不同品系間養殖效益: 比較k105 F1、CP106 F1與選育2代之K103 F3、選育3代之S101高產量F4與S101高產次F4之養殖 成效,在密度200隻/m 2 養殖17週後, K103 F3在產量(kg/m 2 )上與兩批進口種苗 F1無顯著差異,且K103 F3的末重顯著較高,FCR較低,顯示本土選育種原的養殖經 濟效益明顯較佳。

研究報告摘要(英)


The objectives of the project are to establish the SPF broodstock of Litopenaeus vannamei and to improve high growth and high reproductive performance through selective breeding. The results of this year were following: 1. Introduce of broodstock: five batches of broodstock were introduced and kept in Tungkang Biotechnology Research Center, Taiwan Fisheries Research Institute. 2. Diseases diagnose: broodstock were screened with WSSV, TSV, YHV/GAV, IMNV and IHHNV virus, EMS pathogen, Vibrio parahaemolyticus and EHP, microsporidian parasite using qPCR . 3. Selection for high reproductive performance: the number of spawn were highly variable within 130 females used in this study, in which, 9 no spawn at all; 21 spawn more than 11 times, which spawn 298 times in total and represents 40% of the grand total of 741 times. 4.Selection for high growth: This study using the offspring of three batches with high reproductive performance to go through 3-step high growth selection program. The selective pressure for the first and the second step of selection were 22-34% and 25-30% for male, 23-29% for female, respectively. 5.The efficiency of selective breeding: The strain of S101 high reproductive times had better selection response and realized heritability, 7.13% and 0.24, respectively. 6.Culture performance with different strains: Compared culture performance between local breeding (2-3generation) and F1 of imported broodstock or imported PL5-10 at density 200/m2 for 17 weeks. The results showed k103 F3 had the as great yield (kg/m2) as the two strain of imported stocks. In addition, the final weight of k103 F3 was significantly higher (P <0.05) and the FCR was lower, indicating that the domestic breeding germplasm culture benefit is obviously better.