農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

本研究供試藻株有1株聚球藻(Synechococcus sp.)、1株海水綠球藻 (Chlorella sp.french )、1株擬球藻(Nannochloropsis oceanica)、1株杜氏藻 (Dunaliella primolecta)、1株球形四鞭藻(Carteria globosa)、1株周氏扁藻 (Tetraselmis chui)、1株扁藻(TetraselmisR. Lewin )、1株亞心型扁藻 (Tetraselmis subcordiformis)、1株司西扁藻(Tetraselmis suecica)及1株四鞭扁 藻(Tetraselmis tetrathele)，供試藻株以綠色鞭毛藻類為主，共10株微藻種原。 探討培養液之配方、鹽度、溫度與光照度對保種之影響，比較施打空氣與二氧化碳 對種原增殖及培養液pH值之影響，建立最佳保種條件。        結果顯示最適保種光照度及溫度聚球藻(MN41)為50umol/m 2 /s及30℃、四 鞭扁藻(TT)為50umol/m 2 /s及25℃，亞心型扁藻(TSC)、司西扁藻(TS)、周氏扁藻 (TC-2)、球形四鞭藻(CAG)及杜氏藻(LB1000)之最適保種光照度為50umol/m 2 /s；LB1000以f/2加簡易綠藻配方(f/2+g)培養有最佳SGR；亞心型扁藻(TSC)、司西 扁藻(TS)、周氏扁藻(TC-2)及杜氏藻(LB1000)以鹽度30-35psu培養可得較佳特殊成 長率；微藻擴增培養試驗，添加微量二氧化碳可促進藻株之特殊成長率，且維持培 養液PH值中性。應用於餌料生物培養，結果顯示以四鞭扁藻(TT)和亞心型扁藻 (TSC)投餵小型輪蟲、四鞭扁藻(TT)投餵極小型輪蟲及司西扁藻(TS)投餵短角異劍水 蚤可獲得最佳SGR；以聚球藻(MN41)投餵三種餌料生物皆產生最差SGR。

This study aims to find optimum stock condition of microalga on strains preservation. Microalga included 1Synechococcus sp.(MN41), 1Chlorella sp. french (CHL-S7), 1Nannochloropsis oceanica (NI),1Tetraselmis chui (TC2),1Tetraselmis suecica(TS),1Tetraselmis tetrathele (TT),1Tetraselmis subcordiformis (TSC),1Tetraselmis R. Lewin (TRL),1Carteria globosa (TSC) and 1Dunaliella primolecta (LB1000). The test strains mainly consisted of green flagellate microalgae, a total of 10 strains of microalgae. The results showed that optimum temperature and photosynthetically active radiation of microalga stock condition are 30℃and 50umol/m2 /s for culturing MN41, 25℃and 50umol/m2 /s for culturing TT; The optimum PAR for keeping TSC、TS、TC-2、CAG and LB1000 are 50umol/m2 /s. The optimum microalga stock mediums is LB1000 in f/2 plus simple formula for green algae. The optimum degrees of salinity is 30-35psu for TSC, TS, TC-2 and LB1000.Microalgae culture experiments, pumping trace amounts of carbon dioxide or air in can promote the growth rate of microalgae, and maintain PH value of the culture medium.The best SGR of results showed that S type rotifer ( Brachionus ibericus ) is fed with TT and TSC, SS type rotifer ( Brachionus rotundiformis ) is fed with TT, the copepod ( Apocyclops royi ) is fed with TT; the worst SGR was found when feeding three kinds of livefoods with MN41.