The Japanese eel is one of the most important economic aquaculture species of east Asia. All seedling used for aquaculture farm is completely dependent on wild glass eels caught in estuaries and coastal areas. However , natural sources of glass eels have drastically declined in recent years. Therefore, it is necessary to establish a method for seed production of eel elvers that is not dependent on wild catch. This study was conducted to explore the suitable methods for induced spawning of the japanese eel ( Anguilla japonica ). Repeated injections ofsalmon pituitary homogenates mixed gonadotropin can induce spermatogensis in male eels and vitellogenesis in female eels, and female eels ovulation can be achieved by injection of 17α,20β-dihydroxy-4-pregnen-3-one (DHP) .This year total of about 120 eels were injected.By body weight gain, the degree of opening of the vent opening and the diameter of the oocysts to determine the timing of oviposition in developing mature female eels, more than 80% of the female eels can induce spawning. At present, the total fecundity is about 3 million, part of the fertilized eggs hatched, eel seed survival to 6 days. This results might suggest When the ratio of oocyte diameter 0.95-1.00 mm more than 50% to induced oviposition, fertilized eggs and hatchling eel seed can be obtained.