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建置微藻保種之標準化技術及其應用

  • 日期:105-06-03
  • 計畫編號:105農科-11.3.3-水-A1(5)
  • 年度:2016
  • 領域:漁業科技研發領域
  • 主持人:周芷儀
  • 研究人員:黃維能、王淑欣

本研究供試藻株有1株骨藻( Skeletonema costatum )、1株海鏈藻( Thalassiosira weissflogii )、1株擬球藻( Nannochloropsis oceanica )、2株周 氏扁藻( Tetraselmis chui)及5株角毛藻( Chaetoceros gracilis ),前者周氏扁 藻為TC1及LB232,後者角毛藻為CG1、CG2、CG3、CG4及CG5,共10株微藻種原。探討 培養液之配方、鹽度、溫度與光照度對保種之影響,並就施打空氣與二氧化碳對藻 種增殖及培養液pH值之影響,建立最佳保種條件。 結果顯示最適保種光照度及溫度擬球藻(NJ)、周氏扁藻(TC1)、骨藻(SKE)及角毛藻 (CG1、CG2)為100umol/m2/s及25℃,周氏扁藻(LB232)、海鏈藻(CY)為100 umol/m2 /s及20 ℃,角毛藻(CG3)為50 umol/m2 /s及15 ℃,角毛藻(CG4)為50 umol/m2 /s及 20-30℃,角毛藻(CG5)為100、50 umol/m2 /s及30℃;角毛藻(CG2)以Walne,角毛藻 (CG1、CG4、CG5)以Walne加簡易綠藻配方,周氏扁藻(TC1)以f/2為最適培養液,骨 藻最適培養液為f/2加簡易綠藻配方;角毛藻(CG2、CG3、CG4)以鹽度25-30psu培養 可得較佳特殊成長率。微藻擴增培養試驗,添加微量二氧化碳或打氣可促進多數藻 株之特殊成長率,且維持培養液PH值。

研究報告摘要(英)


This study aims to find optimum stock condition of microalga on strains preservation. Microalga included 1 Skeletonema costatum (SKE), 1 Thalassiosira weissflogii (CY), 1 Nannochloropsis oceanica (NJ), 2 Tetraselmis chui (TC1, LB232) and 5 Chaetoceros gracilis (CG1, CG2, CG3, CG4, CG5), the microalga strains came from different native place. The results showed that optimum temperature and photosynthetically active radiation of microalga stock condition are 25 ℃ and 100 umol/m2 /s for culturing NJ, TC1, SKE, CG1 and CG2; 20 ℃ and 100 umol/m2 /s for culturing LB232, CY; 15 ℃ and 50 umol/m2 /s for CG3; 20-30 ℃ and 50 umol/m2 /s for CG4; 30 ℃ and 50 or 100 umol/m2 /s for CG5 respectively. The optimum microalga stock mediums are CG2 in Walne, CG1, CG4 and CG5 in Walne plus simple formula for green algae, TC1 in f/2, SKE in f/2 plus simple formula for green algae. The optimum degrees of salinity is 25-30 psu for Chaetoceros gracilis (CG2, CG3, CG4). Microalgae culture experiments, pumping trace amounts of carbon dioxide or air in can promote the growth rate of the majority of strains of microalgae, and maintain PH value of the culture medium.