Monosex or sterile fish is advantageous for fish aquaculture. Therefore, the aim of this study will investigate the control of sex and production of all-female progeny in grey mullet (Mugil cephalus). We proceed the following steps: 1. Culture the masculized grey mullet until mature and promote spermiation. 2. Cryopreservation of grey mullet semen. 3.Checkout the sex of grey mullet offspring to confirm the true neomale. Culture these neomale to mature and propagate all-female progeny. In this study, 14 masculized grey mullet were sustainedand fed with aromatase inhibitor (androstatriendione,20mg/kg feed) from August.The mean weight of masculized grey mullet in August was 347.67 ± 61.49 g. InOctober, the body weight increased to 623 ± 103.6 g. In the control group, the average body weight was 573.86 ± 118.62g in August and increased to 721.53 ±151.4g in October. The body weight of the control group was greater than that of the masculized grey mullet, but there was no significant difference in October. In sperm cryopreservation, after 1 hour of liquid nitrogen preservation, the best result was found in 5% DMSO treatment, 75% sperm with mobility and 75 seconds of swimming time.