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全雌化烏魚種苗繁殖技術之研發

  • 日期:105-06-05
  • 計畫編號:105農科-11.4.3-水-A1
  • 年度:2016
  • 領域:漁業科技研發領域
  • 主持人:陳紫媖
  • 研究人員:李彥宏

單一性別或不孕魚類養殖對產業有極大幫助,能增加養殖效益及產業利益,因此本 計畫以烏魚為試驗魚種,進行性別控制之研究,達到增加產業效益的目標。1. 雄性 化烏魚保種蓄養及促進產精試驗。2.新雄烏魚精子凍結保存。3.確認新雄烏魚,以 人工授精方式並培育魚苗至生殖腺性別分化來確認XX型雄魚。培養新雄魚至性成熟 ,在與一般母魚繁殖,預計可生產全雌化烏魚子代,推廣或技術轉移給養殖業者。 在本研究中,投餵添加甲基睪固酮將烏魚雄性化並持續蓄養,目前有14尾,對照組 有15尾,每尾標示晶片區別。於8月起餵食含有環化酵素抑制劑 androstatriendione(ATD, 20mg/kg feed), 8月雄性化烏魚平均體重 347.67±61.49克, 10月份,體重成長至623±103.6克。對照組8月平均體重 573.86±118.62克, 10月份,體重成長至721.53±151.4克。對照組平均體重大於雄 性化烏魚,但在10月時並無顯著差異。在精子凍結保存測試中,經過1小時液態氮保 存,精子回溫後,加入海水之精子活動比例,以5% DMSO保存效果最好,有75%精子 具活動能力,游動時間達75秒。

研究報告摘要(英)


Monosex or sterile fish is advantageous for fish aquaculture. Therefore, the aim of this study will investigate the control of sex and production of all-female progeny in grey mullet (Mugil cephalus). We proceed the following steps: 1. Culture the masculized grey mullet until mature and promote spermiation. 2. Cryopreservation of grey mullet semen. 3.Checkout the sex of grey mullet offspring to confirm the true neomale. Culture these neomale to mature and propagate all-female progeny. In this study, 14 masculized grey mullet were sustainedand fed with aromatase inhibitor (androstatriendione,20mg/kg feed) from August.The mean weight of masculized grey mullet in August was 347.67 ± 61.49 g. InOctober, the body weight increased to 623 ± 103.6 g. In the control group, the average body weight was 573.86 ± 118.62g in August and increased to 721.53 ±151.4g in October. The body weight of the control group was greater than that of the masculized grey mullet, but there was no significant difference in October. In sperm cryopreservation, after 1 hour of liquid nitrogen preservation, the best result was found in 5% DMSO treatment, 75% sperm with mobility and 75 seconds of swimming time.