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褐藻作為改善肌肉流失之營養膳食補充品研發(3)

  • 日期:110-07-30
  • 計畫編號:109農科-3.5.2-水-A1
  • 年度:2020
  • 領域:食品科技研發
  • 主持人:蔡慧君
  • 研究人員:易琮凱、杜明杰

250 μg/ml 冬青葉馬尾藻(Sargassum ilicifolium)與中國半葉馬尾藻(Sargassum hemiphyllum var. chinense)水解物對葡萄糖醣皮質類類固醇藥物(如 Dexamethasone)具有較好的緩解能力,在兩者以3:0;2:1與1.5:1.5混合作用下,可 以觀察到小鼠骨骼肌細胞(C2C12)中,受Dexamethasone影響,mRNA表現量降低的生 長因子AKT1、PI3K與TRPV4有回復至正常水平的趨勢,其中以3:0比例添加時,具有 較顯著的結果,綜合評估後以冬青葉馬尾藻水解物做為後續實驗及產品開發的原料 。口服急毒性分析結果顯示食用5g/kg冬青葉馬尾藻萃取物的試驗鼠無慢性中毒或死 亡現象;基因毒性試驗則顯示該試驗物質與其代謝後的物質對測試菌株並不具有致 變異性。褐藻膳食補充品與市售產品的比較試驗中,在肌肉生長下游指標4EBP1、 S6K1與mTOR方面,僅一款B產品在4EBP1表現上提升至111.47%具有較高的表現量,在 S6K1與mTOR mRNA表現量上與各市售品間無顯著差異,在緩解Dexamethasone肌肉流 失狀況的試驗中,Trpv4與 PI3K表現量以冬青葉馬尾藻膳食補充品效果最好, Trpv4 mRNA表現量由0.70倍→1.60倍,PI3K mRNA表現量由0.75倍→2.05倍,可促進 肌肉生長緩解類固醇藥物造成的肌肉萎縮。綜合以上試驗, 冬青葉馬尾藻水解物可 改善肌肉流失,作為營養膳食補充品,能協助緩解肌少症的現象。

研究報告摘要(英)


The addition of 250 μg/mL of Sargassum ilicifolium and Sargassum hemiphyllum var. chinense hydrolysate to mouse skeletal muscle cells C2C12 can mitigate sarcopenia side effects from glucocorticoids. As the ratio between S.ilicifolium and S.hemiphyllum var. chinense are 3:0, 2:1 and 1.5:1.5, we notice the mRNA expression of AKT1, PI3K and TRPV4 reduce by Dexamethasone in C2C12 have a tendency to return to normal levels. Considering when the ratio of S.ilicifolium and S. hemiphyllum var. chinense is 3:0, it has more significant results, we finally decide to use S. ilicifolium as material of product development. The result of acute oral toxicity analysis shows no chronic poisoning or death occurred in the experimental mice consuming 5 g/kg of S.ilicifolium extract; Ames test shows no mutagenicity in S.ilicifolium and its metabolite. Comparing with commercially products, in the mRNA expression of downstream indicators of muscle growth: 4EBP1, S6K1, and mTOR, only the the product of B increases the 4EBP1 mRNA expression to 111.47%. There are no significantly difference in S6K1 and mTOR mRNA expression among brown algae dietary supplement and commercial products. Adding Dexamethasone and different nutritional supplements in the culture of C2C12, observe the growth factor mRNA expression between S.ilicifolium nutritional supplement and other commercial products. We find only the S.ilicifolium hydrolysate increase the mRNA expression of Trpv4 (0.70→1.60 fold of control) and PI3K (0.75→2.05 fold of control).It means contrast to other commercial products, brown algae dietary supplement has better performance at promoting muscle growth and mitigating sarcopenia side effects. Above all, S.ilicifolium hydrolysate nutritional supplement can improve muscle loss, and mitigating symptom s of sarcopenia.