The addition of 250 μg/mL of Sargassum ilicifolium and Sargassum hemiphyllum var. chinense hydrolysate to mouse skeletal muscle cells C2C12 can mitigate sarcopenia side effects from glucocorticoids. As the ratio between S.ilicifolium and S.hemiphyllum var. chinense are 3:0, 2:1 and 1.5:1.5, we notice the mRNA expression of AKT1, PI3K and TRPV4 reduce by Dexamethasone in C2C12 have a tendency to return to normal levels. Considering when the ratio of S.ilicifolium and S. hemiphyllum var. chinense is 3:0, it has more significant results, we finally decide to use S. ilicifolium as material of product development. The result of acute oral toxicity analysis shows no chronic poisoning or death occurred in the experimental mice consuming 5 g/kg of S.ilicifolium extract; Ames test shows no mutagenicity in S.ilicifolium and its metabolite. Comparing with commercially products, in the mRNA expression of downstream indicators of muscle growth: 4EBP1, S6K1, and mTOR, only the the product of B increases the 4EBP1 mRNA expression to 111.47%. There are no significantly difference in S6K1 and mTOR mRNA expression among brown algae dietary supplement and commercial products. Adding Dexamethasone and different nutritional supplements in the culture of C2C12, observe the growth factor mRNA expression between S.ilicifolium nutritional supplement and other commercial products. We find only the S.ilicifolium hydrolysate increase the mRNA expression of Trpv4 (0.70→1.60 fold of control) and PI3K (0.75→2.05 fold of control).It means contrast to other commercial products, brown algae dietary supplement has better performance at promoting muscle growth and mitigating sarcopenia side effects. Above all, S.ilicifolium hydrolysate nutritional supplement can improve muscle loss, and mitigating symptom s of sarcopenia.