This project first explores the effect of attaching seedlings of artificial attachment base to understand the difference between artificial materials and oyster shell. The attachment bases made up of 10 strands of PP, PVC, PLA, bricks and cement blocks were placed vertically to attach seedlings to oysters. After 72 hours, the amount of attached seedlings on each attachment base was recorded. The results showed that the effect of attaching seedlings was oyster shell> PLA> PP> PVC> bricks> cement, and the attachment amount was 38.45±12.31, 22.26±8.41, 18.37±11.25, 13.54±5.84, 10.11±5.54 and 3.28±1.25 oyster seedlings/Sheet, it can be seen that although several attachment bases (especially PP and PLA) are not as effective as oyster shells, they still have potential for development. In addition, this project intends to explore the influence of ocean acidification(OA) on Taiwan-produced oyster planktic larval stage. The pH value is set to a lower seawater environment (pH 7.6, 7.8, 8.0) using CO2 aeration method, and the hatching rate of oyster eggs is observed. And whether there is a difference in the mortality rate and growth rate of the oyster larval. The results showed that the hatching rates of pH7.6, 7.8, 8.0 and control groups were 6.41±2.14%、12.38±2.65%、10.07±1.95% and 11.08±3.68. The pH 8.0 group and the control group have a significantly 6-15% larger body size than the pH 7.6 group from incubation to 7-10 days. Only the survival rate shows a large change and no significant trend. It can be seen that the water environment as low as pH 7.6 has a negative impact on the early stage of oyster larval development. As the ocean tends to be acidified and cannot be stopped, attention should be paid to the impact of OA on the oyster seedling industry.