跳到主要內容區塊

建立法囊藻培育條件及其粗萃取液抗氧化能力評估

  • 日期:110-08-02
  • 計畫編號:109農科-9.3.3-水-A1(3)
  • 年度:2020
  • 領域:漁業科技研發
  • 主持人:李沛珊
  • 研究人員:黃君毅

法囊藻的地理分佈非常廣泛,並且已被廣泛應用於研究跨生物膜的離子交換和水勢 。但是,到目前為止,他仍僅止於收集及少數食用,並無有關培養和利用的文獻發 表。此計畫旨在建立法囊藻生長條件及分析抗氧化能力。不僅可為產業提供原料 ,還可為量產技術做準備。首先,本計畫探討其成長條件,試驗結果發現,水溫為 20℃、光照強度為3,000-6,000 lux、初始培養密度為0.25 g、鹽度為35 psu、海水 +1 ppm銨鹽+0.01 ppm磷酸鹽及採固定在蘭花網上方式,為法囊藻小量室內培養最佳 條件。接著通過分析其抗氧化能力,發現粗萃取物的最終體積為室溫組94 ml、加熱 組91 ml、高溫高壓組89 ml及液態氮組92 ml,且得知高溫高壓組之粗萃取液其總醣 含量 (1.69±0.03 mg/ml葡萄糖)、總酚含量 (56.62±0.51 μg/ml沒食子酸)、還原 力 (30.59±0.93 μg/ml維生素C)、清除超氧陰離子能力 (40.02±1.25%) 及捕捉 DPPH 自由基能力 (70.55±0.91%) 皆為最佳,只有螯合亞鐵離子能力是以室溫組 (82.65±0.55%)最好。經由上述研究,我們發現法囊藻粗萃取液部分抗氧化特性非常 好,不失為一個良好、健康及安全的抗氧化素材。

研究報告摘要(英)


Valonia aegagropila has a very wide geographical distribution and has been widely used to study ion exchange and water potential across biofilms. However , up to this date, it is still only based on collection, and there is no literature on cultivation and utilization. This project intends to establish the growth conditions and analyze the antioxidant capacity of Valonia aegagropila. Not only can provide raw materials for the industry to use, but also prepare for mass production technology. First, we discussed its growth conditions. The results of the experiment were that the optimum temperature  is 20 ℃, the light intensity is 3000-6000 lux, the initial cultivation density is 0.25 g, the salinity is 35 psu, and the nutrient salt is sea added with 1 ppm ammonium salt and 0.01 ppm phosphate and culture method are fixed on orchid net (without aeration) as the best culture conditions. After analyzing its antioxidant capacity, we found that the final volume of the crude extract was 94 ml in the room temperature group, 91 ml in the heating group, 89 ml in the high temperature and high pressure group, and 92 ml in the liquid nitrogen group. Regardless of the total sugar content (1.69±0.03 mg/ml glucose), total phenol content (56.62±0.51 μg/ml gallic acid), reducing power (30.59±0.93 μg/ml vitamin C), the ability to scavenge superoxide anion (40.02±1.25%) and capture DPPH free radicals (70.55±0.91%) are the best in the high temperature and high pressure group. Only the ability to chelate ferrous ions is the best in the room temperature group (82.65±0.55%). We found that some of its antioxidant properties are very good, and it is a good, healthy and safe antioxidant material.