The broodstock of Plectropomus leopardus was divided into four ponds (all without hormone induced) for mating and spawning. On May 4, the broodstock was moved to the outdoor pond and moved to the indoor spawning pool on May 13. The Spawning period of the broodstock was from May 15 to Oct 7 and lasted 146 days, including 113 spawning days. The total egg weight was 36,342 g(7,268.4×104 eggs),including the buoyant eggs(23,928g; 4,785.7×104 eggs; 65.84%) and the sinking eggs (12,413g; 2,482.7×104 eggs; 34.16%). Mean egg weight was 322±327 g(64.4×104 eggs),and the highest number of daily spawning was 1,560g(312.0×104 eggs). During the period, the water temperature was 25.4-30.9℃. The results showed that the spawning pond with longer photoperiod was better than the one with shorter photoperiod. We collected fertilized egg samples of P. leopardus to detect for NNV and GIV. The result of NNV RT-PCR test of fertilized eggs showed that the ratio of positive was 0% (0/322). The result of GIV PCR test of fertilized eggs showed that the ratio of positive was 0% (0/322). Using ozone and sintered oyster shell powder to treat the circulating water in model factory for P. leopardus can kill some bacteria and achieve the effect of reducing bacteria. However, the usage of ozone and sintered oyster shell powder must be confirmed by further test before it can be used safely.