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強化午仔魚腸道健康之機能飼料研發

  • 日期:110-08-02
  • 計畫編號:109農科-9.5.7-水-A2
  • 年度:2020
  • 領域:漁業科技研發
  • 主持人:黃淑敏
  • 研究人員:黃美瑩、周瑞良、劉璟儀

午仔魚(Eleutheronema tetradactylum)為臺灣最重要的海水魚類之一,並為臺灣沿 海漁業帶來了巨大的經濟價值。 過度餵食雖然能將生長期縮短,然而對魚類之消化 道產生高緊迫,進而造成腸道疾病頻繁發生。 本研究目的為評估益生菌合併商業飼 料之口服應用於午仔魚之成長與腸道抗菌效果。選擇了三種益生菌菌株,即解澱粉 芽孢桿菌(Bacillus amyloliquefaciens),乳酸菌(Lactobacillus Paracasei )和 葡聚糖的乳酸桿菌(Leuconostoc mesenteroides B4),以每日早晚口服餵食(占飼料 比5%)經餵飼8週並對其生長率,存活率,飼料效率和飼料轉化率進行了效益評估。 試驗結果顯示,在成長率、飼料轉換率與活存率之結果顯示,各組飼料益生菌添加 組與對照組並無統計上明顯差異。此外,在口服益生菌對消化道分離出的病原體於 體內抗菌力之評估試驗,結果顯示僅有口服Ba之益生菌組經高菌數之海豚鏈球菌攻 毒後其存活率尚有48%,顯著高於對照組之存活率24% (p<0.05);以低劑量攻毒對照 組之臟器分離率較高劑量之對照組為高,顯示低濃度之鏈球菌因發病病程延長,導 致體內病原菌之增長; 腸道病變之結果顯示,各益生菌飼料添加組之腸道粘膜上皮 之病變皆較對照組為輕微,顯示在腸道仍有相當之防護能力。本研究可作為益生菌 與商業飼料合併使用之口服效果提供了保護力之評估方法,並可能有助於優化使用 益生菌作為飼料添加劑來替代抗生素,進而提高國內午仔魚之生產安全與品質。

研究報告摘要(英)


The fourfinger threadfin (Eleutheronema tetradactylum) is one of the most important marine fish species, that contributes great economic values to Taiwanese coastal fisheries. Over-feeding results in the shorten growing period, however that induces the high stress in digestion tract of fish, and causes the intestinal diseases to occur frequently. The objectives of this study were to evaluate the effects of probiotic in application with the commercial feed in fourfinger tetradactylum. Three probiotic strains, Bacillus amyloliquefaciens, Lactobacillus Paracasei  and Leuconostoc mesenteroides B4, were selected and evaluated on growth rate, survival rate, feed efficiency and feed conversion. The results of growth rates, feed conversion rates and survival rates showed that there was no statistically significant difference between the feed probiotic supplementation groups and the control group. The survival rates of the probiotic feed with Ba group was 48% after being challenge with the high dose Streptococcus iniae which was significantly higher than the 24% of the control group (p<0.05). The low-dose challenge control group has higher organ isolation rates than the higher-dose control group, which shows that the low-concentration streptococcus has a prolonged disease course, leading to the growth of pathogenic bacteria in the fish organs.The results of intestinal lesions showed that the lesions of the intestinal mucosal epithelium of the probiotic feed supplementation groups were all milder than those of the control group, indicating that the test groups still have considerable protective ability in the intestines. This study provides a assessment methods of the role of probiotics in the diet and may help optimize the use of probiotics as feed additives to replace antibiotics, thereby achieving the high safety and quality of production in fourfinger tetradactylum.