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水產加工剩餘物應用於餌料生物之培養

  • 日期:110-08-02
  • 計畫編號:109農科-15.2.2-水-A1(2)
  • 年度:2020
  • 領域:農業資源循環產業創新
  • 主持人:郭裔培
  • 研究人員:楊順德、劉恩良、溫鈺涓

本計畫過去 2 年度已完成吳郭魚加工剩餘物的水解技術建立與光合菌的分離純化 ,本年度結合水解與光合菌兩項技術,進行餌料生物生產與魚苗培育試驗。體外抑 菌試驗結果顯示,本中心自行分離的沼澤紅假單孢菌 (Rhodopseudomonas palustris, EL strain)  光合菌能有效競爭性抑制嗜水性單胞產氣菌 (Aeromonas hydrophila)、無乳鏈球菌 (Streptococcus agalactiae) 及瓶鼻海豚鏈球菌 (S. iniae),且能應用於輪蟲、饒足類與水蚤等多種的餌料生物培養,滿足魚苗各發育 階段所需。次世代定序分析不同方法培養的餌料生物菌相,與傳統自然發酵法 (對 照組) 相比,以水解魚蛋白結合光合菌培養餌料生物 (試驗組),能顯著降低單胞產 氣菌科的帶原量,與體外抑菌結果相符,證實本方法確實能降低病原菌帶原的風險 ;非度量多維度分析定序結果,試驗組的組間菌相差異較對照組低,顯示以光合菌 生產餌料生物,能有效控管菌相組成,確保餌料生物的品質穩定性。一般成分方面 ,試驗組的粗蛋白質含量顯著高於對照組,且粗脂質含量顯著降低。為比較 2 種方 法生產的餌料生物實際應用於魚苗培育的效益,本計畫進行 25 天的銀鱸苗培育試 驗,試驗組的活存率與成長表現顯著高於對照組 20%,驗證本方法不但能達到加工 剩餘物的循環再利用,對魚苗培育亦有正面效益。

研究報告摘要(英)


Last 2 year, we have established tilapia by-product hydrolysis procedure and isolated photosynthetic bacteria (PSB) in this project. This year, we combined hydrolysis and PSB technologies to produce live feed for fish fry culture trial. In vitro results of pathogen inhibition showed isolated PSB (Rhodopseudomonas palustris, EL strain) had great effect of competitive inhibition against Aeromonas hydrophila, Streptococcus agalactiae and S. iniae. Furthermore, PSB can also be applied on mutiple live feed culture, such as Brachionus sp., Cyclops sp. and Daphnia sp., offering different development stage fish fry. Next-generation sequencing results demonstrated comparing control group (natural fermentation method), experimental group (fish protein hydrolysate (FPH) combined PSB) had significantly lower relative abundance of Aeromonadaceae, which is consistent with In vitro results. It validated FPH combined PSB method can reduce the pathogen-carrying risk of live feed.  Moreover, non-metric multi-dimensional scaling (NMDS) results showed within-group variance in experimental group was lower than in control group, indicating bacteria flora can effectively controled, and ensure the stability of ive feed quality. In aspect of proximate composition, the experimental group had significantly higher crude protein content, but significantly lower crude lipid. To evalute the benefits of these 2 live feed producrion method to practical fish fry culture, we conducted Bidyanus bidyanus fry feeding experiment for 25 days. The survival rate and growth performance of experimental group were significantly higher than that of control group by 20% approximately, validating the live feed culture method combined FPH and PSB not only can promote the reuse of fisheries by-product , but also has benefits on fish fry culture.