At present, the institute has established the cultivation technology of Botryocladia leptopoda, but the corresponding processing technology is lacking. In the last year's research, it was found that the polyphenol from B. leptopoda has strong antioxidant capacity and has the potential for wound repair. Therefore, this study intends to evaluate its repairing effect on damaged skin tissues with the functional components of B. leptopoda, and develop it as a skin care product year by year, which will be used in daily maintenance and postoperative maintenance of medical cosmetology to increase the demand and economic value of B. leptopoda. Add 250 μg/ml FE or 250 μg/ml AE into the culture medium of the L929 cells 24 hours can promote the value of L929 proliferation rising to 324% or 212%, and if compounding the phenol with Ax, the value of cell proliferation is about 5 times longer, which showing the synergistic effect can accelerate the speed of damage repair. Using 1 μg/ml PMA induces the expression of MMP1 and MMP2 mRNA rise to 1.7 and 5.4-fold of the control, then add 250 μg/ml FE can respectively reach 33.4% and 48.7% ability of inhibition; add 250g/ml AE can inhibit the MMP2 mRNA expression by 82.8%. The ingredient in B. leptopoda can stabilize the action of metal matrix protease, and can prevent the wound tissue from proliferating too slowly or too quickly. This technique is competitive in the medical cosmetology or skin care market.