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長松藻酵素水解活性成分及其應用

  • 日期:111-10-20
  • 計畫編號:110農科-6.3.1-水-A1(2)
  • 年度:2021
  • 領域:漁業科技研發
  • 主持人:邱韻霖
  • 研究人員:林慧秋、廖紫嬿、許雅筑

長松藻(Codium cylindricum)為常見松藻屬綠藻,產季為每年4-7月,澎湖周邊海域每年會出現大量長松藻。文獻指出長松藻具有抑制肥胖,炎症和血管生成的作用。但是,關於其體內生物利用度和生物轉化的信息卻很少。因此,本中心將進行其有效成分分析及利用。結果顯示,一般成分以水分92.76±0.12為最高,重金屬:總砷2.04 ppm,鉛0.06 ppm,鎘及汞未檢出。胺基酸含量以麩胺酸154.72 mg/100g最高,其次為天門冬胺酸、白胺酸、丙胺酸、離胺酸。脂肪酸含量以棕櫚酸29.03% 其次是α-次亞麻油酸21.41%。比較特殊的是γ-次亞麻油酸、α-次亞麻油酸及二十碳四烯酸等多元不飽和脂肪酸,是調節人體生理機能的重要活性物質。長松藻使用Viscozyme、 Everlase、 Flavourzyme、 Pepsin、 Celluclast 5種酵素水解及水萃取,其總醣含量以Viscozyme酵素水解,在各個濃度總醣含量均為最高。硫酸根含量分析方面,無論分子量在3kDa以上或以下,皆以水萃取為最高,在濃度1200mg/ml硫酸根含量可達
269.58±0.52μg/ml,其他組則較低。類胡蘿蔔素含量分析,結果顯示水萃取2.31±0.40mg/ml及Viscozyme水解 1.88±0.25 mg/ml含量較高,其他酵素水解則含量較低。利用長松藻特殊之活性物質,可運用於食品天然抗氧化劑、抗菌劑、製藥及化妝品工業等,值得後續研究開發。

研究報告摘要(英)


Codium cylindricum, a common green algae of the genus Codium, is abundant in the surrounding waters of Penghu, especially in April to July. Previous literatures indicate that Codium cylindricum could inhibit obesity, inflammation and angiogenesis;
however, the information about bioavailability and biotransformation is deficient. Therefore, we analyzed and utilized the active ingredient of it. The result showed that it comprises 92.76±0.12 of water. The result of heavy metal test was as follow: 2.04 ppm of total arsenic, 0.06 ppm of lead, cadmium and mercury undetected. The content of amino acid was as follow in order: Glutamic acid (154.72 mg/100g), Aspartic acid, Leucine, Alanine, and Lysine. The content of fatty acid was as follow in order: 29.03% of Palmitic acid and 21.41% of α-Linolenic acid (ALA). In addition, it also contains Polyunsaturated fatty acid, such as γ-Linolenic Acid, α-Linolenic acid, and eicosatetraenoic acid, which could regulate human physiological functions. The results of water extraction and enzyme hydrolysis with Viscozyme, Everlase, Flavourzyme, Pepsin, and Celluclast are as follow. The highest values of total sugars content were obtained from enzyme hydrolysis with Viscozyme. The higher values of sulfate content were obtained from water extraction whether molecular weight is over or below 3000 kDa; moreover, the highest value is 269.58±0.52μg/ml under a concentration of 1200mg/ml. The top two values of carotenoid content were obtained from water extraction (2.31±0.40mg/ml) and enzyme hydrolysis with Viscozyme (1.88±0.25 mg/ml). The bioactive material of Codium cylindricum could be used as natural food antioxidant and antimicrobials, and furthermore, be used in pharmaceutical and cosmetic industries. It worth further study and development.