In this study, we collected Pleuronectiformes in the coastal waters around Taiwan this year, including Leopard flounder (Bothus pantherinus), Shallow-water sole (Sy naptura marginata), Olive flounder (Paralichthys olivaceus), Lndian halibut (Psett odes erumei), and Zebra sole (Zebrias zebra) and Peacock sole (Pardachirus pavoninus). fish culture in running water ponds and the temperature of the water are controlled at 22±1℃. Feeding krill and clove fish can be stably cultured. In addition, conduct breeding temperature and light experiments. The fish culture with four temperature conditions of 18℃, 22℃, 26℃ and 30℃；three lighting conditions of 200-300 lux, 800-1000 lux and 1500-2000 lux, respectively for 8 weeks. Both leopard flounder and olive flounder have the best weight gain rate at a water temperature of 26°C; they have the best weight gain rate under cultivation under 800-1000 lux light.