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鯖魚頭開發貓類機能性飲品之研究(II)

  • 日期:110-08-02
  • 計畫編號:109農科-12.1.4-水-A1
  • 年度:2020
  • 領域:農業生物經濟
  • 主持人:高翊峰
  • 研究人員:羅子鈞

隨著國人飼養家貓作為伴侶動物的風氣日漸增加,家貓的保健營養亦逐漸受到重視 。本計畫先前已完成水產磷脂質對家貓免疫調節確效分析,本年度分別以安姆試驗 (Ames test)、細胞毒性分析(cytotoxicity)、口服急毒性試驗(acute oral toxicity)及呼吸急毒性分析(acute inhalation toxicity)分析水產磷脂質之使 用安全性。實驗結果顯示:水產磷脂質(鯖魚頭磷脂質) 在5 mg/plate濃度下,無 論有無經過大鼠肝臟代謝酵素活化過程,五株試驗菌株包含TA97a、TA98、TA100、 TA102 及TA1535 之回復突變菌落數與負對照組相比較皆未達陽性判定標準。在細胞 實驗中,添加3 mg/mL以下的水產磷脂質(鯖魚頭磷脂質)對家貓初代培養週邊血液 單核球細胞無明顯毒性;口服急毒性試驗:單次給予口服限定劑量每公斤體重5000 mg/kg之水產磷脂質(魷魚皮磷脂質),試驗48小時對對雌大鼠(SD品系)無明顯及 毒性,第14天經安樂死犧牲後,經大體解剖肉眼觀察臟器亦無明顯的外觀病變;呼 吸急毒性試驗:大鼠經鼻式呼吸暴露於3.23±1.27 mg/L之氣膠態水產磷脂質(鯖魚 頭磷脂質)連續處理4小時,續觀察14天,試驗動物全部活存,在第14天經安樂死處 理後剖檢,部分動物肺臟偶發非專一性白色突起或暗紅色斑點/斑塊,肺泡組織切片 無明顯病變。綜合上述試驗結果輔以美國食品藥物管理局相關認定,水產磷脂質應 屬公認安全性物質(Generally Recognize as Safe),應可廣泛運用在食品或伴侶 動物保健產品市場的開發上。

研究報告摘要(英)


The domesticated cat keeping has found dramatic rise in the past decade. Hence, the healthcares and nutrition supplements of market on the domestic feline have gradually received attention. In the 2019, we have createded a functional phospholipid component (MH-Lip.) from mackerel processing byproduct (head) as well as squid byproduct (integument). The MH-Lip. functions have been confirmed the inflammatory regulation effects on a feline primary culture cell. In this year, the Ames test, cell cytotoxicity test, acute oral toxicity test and acute respiratory toxicity analysis of marine phospholipids (from the squid integement and the mackerel head) have further been conducted for evaluation of the biosafty on feline nutrition intake. As the results, compared to the negative control group, the number of colonie at the five test strains included TA97a, TA98, TA100, TA102 and TA1535 did not reach the positive criterion on the MH-Lip treatment group at a concentration of 5 mg/plate. In the cytotoxicity of MH-Lip., adding less than 3 mg/mL MH-Lip. has no obvious toxicity on the peripheral blood mononuclear cells of domestic cats primary culture. In the experiment of an acute oral toxicity, oral administration of single dose (5000mg/Kg of rat weight) SQ-Lip. showed no acute toxic effects on female rats (SD strain) after 48 hours of test. On the 14th day, the sacrificed experimental rat organs were not observed the obvious appearance lesions at the all treatment rats. In the experiment of acute inhalation toxicity, the rats were exposed to 3.23±1.27 mg/L of aersol MH-Lip. through nasal breathing for 4 hours, followed by observation for 14 days. All the animals were alive and were sacrificed after euthanasia on the 14th day. Some animals had occasional non-specific white protrusions or dark red spots/plaques in their lungs, and alveolar tissue sections showed no obvious lesions. Based on the above test results and the relevant bio-safty certification from the US Food and Drug Administration, the MH-Lip. should be the generally recognized as safe substances (GRAS) and should be widely used in the development of food or the companion animal health products markets.