The study aimed to develop Ga, In, and Mo analysis methods in aquatic animals. Several criteria such as linearity, limits of quantification (LOQ), specificity, precision and accuracy were evaluated. Results indicate that this method could be used for the routine determination of Ga, In, and Mo in aquatic animals. A total of 50 samples of various types of aquatic products collected from the test pool were analyzed. The average content of testing heavy metals, such as lead, cadmium, and mercury, were ND-0.37, ND-0.17, and ND-0.01 mg/kg. In addition, indium, gallium and Molybdenum were not detected. By the risk assessment, it shows that the amount of heavy metals in aquatic animals all meet the safety range. Although some aquatic products still have a small amount of heavy metals, but as long as a balanced diet is used, excessive intake of harmful heavy metals can be avoided.