1.In the grouper test, In this study we want to collect the Epinephelus moara which is valuable grouper strain, and to establish the breeding technique. In the end, we wish we can set up the cross breeding technology of Epinephelus moara, Epinephelus lanceolatus Epinephelus fuscoguttatus and Epinephelus coioides, and to produce the hybrid strain that can tolerate lower temperatures, fast growing or disease resistant grouper fry, to promote the grouper culture. In this year, we’ve collected 50 Epinephelus moara, 50 genetic data and performed cross breeding experiment for 2 times. We collected the insemination rate and hatching rate of cross breeding experiment, application for grouper farming industry. We have 100 grouper fry of Epinephelus moara and Epinephelus coioides currently in breeding, the average body length of fry is about 15-20cm, the average body weight of fry is about 80-120g.
2.In the tilapia test, the survival rate of each group was 43.5% (control group:) ,100% (the fatty acid supplemented group), 92.6% (fatty acid supplemented vitamin C group). The survival rate of the experimental group was more than 2 times higher than that of the control group. The percentage of the concentration of n3HUFA in the muscles of each group as a percentage of the analyzed total fatty acid concentration: control group: 7.93%; fatty acid supplemented group: 11.17%; fatty acid supplemented vitamin C group: 11.40%. Feeding tilapia fish with unsaturated fatty acid feed can make the survival rate of tilapia fish under low temperature (9℃~10℃/6 hours) higher than that of the control group by more than 2 times. The proportion of n3HUFA in fish is also more than 3% higher than that in the control group.
3.In the white shrimp test, the salty stress and challenge of Vibrio Harvey was carried out in 2 white shrimp family line in the Tungkang Biotechnology Center. In terms of salinity stress, when the salinity was reduced from 30 ppt to 15 ppt, the survival rate of syndicate 2 white shrimp was 100%, and the survival rate of syndicate 1 white shrimp was 92% to 85% on the 7th day after treatment. In addition, after the urgency of salinity, the survival rate of the white shrimp attacked by Vibrio Harvey, the group 2 (17.5%) is better than the group 1 (6%). Results show that the selective breeding of white shrimp can increase the resistance of salinity stress and vibrio challenge. It will alleviate the plight of the white shrimp farming industry.
4.In the clam test, to simulate the double stresses test with high water temperature difference of 7°C (measured at 28-35°C in Changhua) after the salinity of the clam pond dropped sharply during heavy rainfall in summer. Cultured clams (300 clams/600g) and field-collected clams (15- 30 clams/600g) after being acclimatized in 32 ppt seawater (control group) for one week, seawater salinity were dropped to 16, 8, and 4 ppt each for 3 repeats. The salinity was maintained for a week with the above-mentioned high temperature difference. One week later, results showed that the mortality rate of cultured clams held in salinity 4 ppt was as high as 95% and 8 ppt was 20%, which was significantly higher than the control group's 3.3% of 32 ppt and 1.7% of 16 ppt. The mortality rate of field-collected clams after a week of salinity of 4 ppt was 23.3%, 8 ppt was 16.7%, 16 ppt was 10%, and 32 ppt was 3.3%. The experiment measured the lethal salinity conditions of clams after a week of simulating heavy rainfall and restored the summer high temperature climate pattern, which increased the importance of clam farmers to control temperature and salinity through breeding management. Demonstrative IOT (Internet of Things) equipment for clam farming has attracted the willingness of surrounding farmers to adopt it. They has received subsidies for smart farming equipment and invested in a scientific farming management model.