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解析微生物製劑對文蛤腸道菌組成影響之研究

  • 日期:111-10-20
  • 計畫編號:110農科-6.2.5-水-A1(1)
  • 年度:2021
  • 領域:漁業科技研發
  • 主持人:黃慶輝
  • 研究人員:吳嘉哲

臺灣之文蛤養殖戶習慣使用微生物製劑如市售的益生菌或光合菌等進行養殖,但使用微生物製劑對文蛤腸道整體菌相造成之影響則較少探討,本計畫利用新型定序技術解析文蛤腸道菌組成,飼養時文蛤飼料加入本所研發D5 (Bacillus pumilus D5)冷凍乾燥菌粉,並於固定間隔採取文蛤腸道樣本萃取DNA,並運用細菌16S專用引子進行擴增,並進行奈米孔定序以解析文蛤腸道總體菌相,初步結果顯示,飼養2個月後腸道內之弧菌屬(Vibrio)細菌並無顯著減少(施放前佔比11.70%±8.40%、施放1個月後為10.08%±14.57%、施放2個月後10.21%±10.09%,無顯著差異),也未在定序結果中發現Bacillus pumilus D5之16S序列,參考前人研究,這樣的情況可能因為D5菌相比白蝦較不易於文蛤腸道內繁殖,爰D5菌用於改善文蛤養殖情況時,可能需要較長之施放時間以達較佳之效果。

研究報告摘要(英)


Clam farmers in Taiwan are accustomed to using microbial preparations such as commercially available probiotics or photosynthetic bacteria for cultivation, but the impact of the use of microbial preparations on the overall microbial composition of the clam intestines is less discussed. This project useed a new sequencing technology to analyze The intestinal bacteria composition of clams, the clam feed is added to the freeze-dried bacterial powder D5 (Bacillus pumilus D5) developed by our institute. The intestinal samples of clams are taken at
regular intervals to extract DNA, and the bacteria 16S specific primers are used to amplify. Nanopore sequencing was used to analyze the overall bacterial composition of the clam intestine. Preliminary results showed that there was no significant reduction in Vibrio in the clam gut after 2 months of applying D5 (11.70%±8.40% before, 10.21%±10.09% after), and 16S sequence of Bacillus pumilus D5 was not found in sequencing results. Refer to previous studies, this situation may be due to the fact that D5 are less likely to survive in the clam guts than in white shrimp guts. When D5 bacteria are used to improve the culture of clams, longer casting time may be nessasary to achieve better results.