Kuhlia rupestris is a native freshwater fish in Taiwan, which can be used asornamental fish, food fish and game fish. In order to facilitate the identification of individual fish during the subsequent artificial reproduction test, this study conducted a chip identification test. The total length (TL) and body weight (BW) of the fish undergoing chip identification test ranged from 10.0 to 22.7 cm and from 15 to 227 g, respectively. The relationship between TL and BW was estimated as BW=0.0084×TL3.2528 (R2=0.9829,n=40). One month after the chip was implanted, the mortality rate of the fish and the chip identification drop-off rate were 0%. The ratio of intestinal length to total body length of K. rupestris was less than 1, and the structure of pyloric caeca was observed. At present, there is no special feed for K. rupestris. In this study, three commercial feeds ( A feed, B feed and C feed) were used to feed K. rupestris juveniles. As a result, the weight gain rate of fish fed with C feed was better than those fed with A feed. Then we fed C feed to K. rupestris juveniles at 3 feeding frequencies. The results showed that the weight gain rate of the fish in the three-meals-daily group was significantly higher than that of the one-meal-daily group and the two-meals-daily group.