1.To understand the differences in the abundance and diversity of intestinal bacteria indifferent seasons betweentraditional and interior circulating system.2.Establish the method to increase the resistance to disease and immunity.3.Establish the feeding model for brood abalone.4. To execute artificial reproductions of abalone above 10 times a year and tosupplyzygote and larvae for research purpose requirement.5.A total of 60000 abalones were produced.