Yellowfin porgy (Acanthopagrus latus) was sustained anesthetized with 2-phenoxyethanol, and sampled at 0, 1, 6, 12, 24 h during anesthesia and 24 h after recovery. We found that sampling time and anesthetic do really affect the values of hematological parameters. Most time, the control group had higher average values of hematocrit and hemoglobin than the anesthesia group, but the difference between two groups was not significant except in hematocrit at 24 h during anesthesia. Both sets of values of two groups reached the least level at 12 h, and gradually recovered to starting point. As to serum glucose, the anesthesia group had higher average values than the control group from 1 to 12 h during anesthesia, however, the difference between two groups was significant only at 6 h. The values of serum glucose in the anesthesia as well as the control group decreased from starting point, and never recovered even after 24 h recovering.