This study was to investigate the application of 2-phenoxyethanol (2-PE) in the closed transport system involving black porgy in polyethylene bags. After 24 h sealed packaging, addition of 50-200 ppm. 2-PE reduced the accumulation of total ammonia-nitrogen of sea water in the bags containing fish. However, 2-PE could not prevent the happenings of acidification in sea water of bags. In another experiment, effects of 2-PE on the changes of hematological parameters in blackporgy were examined at 0, 12, and 24 h after sealed packaging. The results indicated that packagingtime and anesthetic did not affect the values of hematocrit, hemoglobin and osmolarty. The value of serum glucose was the only changed parameter during this experiment. The values of serum glucose increased following the duration of packaging time. 2-PE added group had lower average values of serum glucose than the control group, but the differences between two groups were not significant. Considering the effect of 2-PE on the reduction of the accumulation of total ammonia-nitrogen in sea water and serum glucose of fish during sealed packaging as well as its cheap price, 2-PE was recommended for application in transport of fish.