農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

本研究以酵母菌發酵石蓴多醣酵素水解液，探討石蓴 (Ulva lactuca) 生質乙醇之生產條件。石蓴粉末 (5%, w/v) 依序以 0.4 N HCl/121°C/20 min、纖維素酶、α-澱粉酶、以及 MA103 與 MAEF108 粗酵素液處理得到石蓴多醣水解液。石蓴多醣水解液的總糖量為 7.39 mg/ml、還原糖量 8.40 mg/ml、與鹽濃度為 5.0%。石蓴多醣水解液接種 (10%, v/v) S. cerevisiae BCRC21685 於室溫下進行批次發酵 72 h 後，發酵液中之乙醇濃度、乙醇產率、及還原糖利用率分別為 0.91%、19.34 g/100 g石蓴 (粉末)、及 53.50%。石蓴多醣水解液接種 (10%, v/v) S. cerevisiae BCRC21685 進行饋料批次發酵 120 h 後，發酵液中之乙醇濃度、乙醇產率、及還原糖利用率分別為 1.02%、21.76 g/100 g石蓴 (粉末)、與 58.75%。饋料批次發酵可進一步提升自石蓴產製生質乙醇之產率，對於未來拓展海洋生質燃料之發展深具潛力。

The present study investigated the production conditions of bioethanol fermented from the enzymatically hydrolyzed polysaccharides (PS) of Ulva lactuca. U. lactuca powder (5%, w/v) was sequentially treated with 0.4 N HCl at 121°C for 20 min, cellulase hydrolysis, α-amylase hydrolysis, and MA103/MAEF108 crude enzymes hydrolysis. The total sugar content, reducing sugar content, and NaCl content of the U. lactuca PS hydrolysate were 7.39 mg/ml, 8.40 mg/ml, and 5.0%, respectively. After U. lactuca PS hydrolysate was fermented with 10% S. cerevisiae BCRC21685 at room temperature for 72 h, the ethanol concentration, ethanol yield, and reducing sugar utilization of the fermentation solution reached 0.91%, 19.34 g/100 g Ulva powder, and 53.5%, respectively. When the U. lactuca PS hydrolysate was fed-batch fermented with 10% S. cerevisiae BCRC21685 at room temperature for 120 h, the ethanol concentration, ethanol yield, and reducing sugar utilization of the fermentation solution reached 1.02%, 21.76 g/100 g Ulva powder, and 58.74%, respectively. Fed-batch fermentation can further enhance the bioethanol production from Ulva lactuca fermentation with S. cerevisiae.