農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

乳酸菌是現今常見的益生菌，對於養殖生物具有正面的幫助。本研究以乳酸菌粉添加於點帶石斑魚 (Epinephelus coioides) 飼料，探討對於石斑魚成長、腸道內菌相及水質影響之研究。實驗分四組濃度餵食乳酸菌粉 (0%、0.5%、1.0%、2.0%)，實驗結果顯示魚腸道內乳酸菌隨著添加濃度增加而增加，而弧菌及異營性菌數量則有減少的現象。抑菌試驗結果，乳酸菌濃度為106 CFU/ml 以上有抑制溶藻弧菌 (Vibrio alginolyticus) 的能力。成長試驗結果顯示，以添加 2.0% 組及 1.0% 組的魚隻全長分別 18.81 ± 0.86 cm 及 18.52 ± 0.70 cm 與 0% 組 17.68 ± 0.73 cm有顯著差異；體重分別為 69.17 ± 7.12 g 及 64.00 ± 8.73 g 與 0% 組 53.04 ± 9.02 g 有顯著差異，添加2.0% 組及1.0% 組成長較快。試驗期間的水質變化，四組濃度組間無明顯差異。在養殖過程中，在飼料中添加 1.0% 乳酸菌粉，乳酸菌即可於腸道環境中生存，減少病原菌量，對於魚隻的成長有正面的助益。

Lactic acid bacteria, Lactobacillus spp. (LAB) are a group of probiotics which are often practiced in aquaculture to enhance growth of aquatic species. In this study, we tried to investigate the effect of powdered LAB added diets on microflora of intestines and on growth of orange-spotted grouper, Epinephelus coioides. After 28 days of feeding, the total numbers of vibrios and LAB were counted by plate colony count and the growth was measured. The results showed that the numbers of LAB of intestines increased with dosage. The number of vibrios reduced owing to the inhibitory effect of LAB. The groupers that were fed with 2.0% and 1.0% of powdered LAB added grew faster than others. In addition, LAB did not affect water quality. Base on the study, it was suggested that a suitable amount of 1.0% LAB added diets could be used in grouper culture to have better growth.