農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

本研究探討Bacillus licheniformis FRI MY-55對於胃酸與膽鹽之耐受情形，並探究點帶石斑 (Epinephelus coioides) 分別飼餵 (1) 對照組、(2) 添加B. licheniformis FRI MY-55 (10⁷ CFU/g)＋乳果糖 (0.15%) 的培養液及 (3) 添加B. licheniformis FRI MY-55 (10⁷ CFU/g)＋果聚糖 (0.15%) 的培養液飼料10週，對於魚隻成長、消化酵素活性及腸道細菌數之影響。結果顯示，B. licheniformis FRI MY-55可以耐受pH 2.5及 1.0% 膽鹽。點帶石斑經分別餵食 (1) 對照組、(2) 添加B. licheniformis FRI MY-55＋乳果糖的培養液及 (3) 添加B. licheniformis FRI MY-55＋果聚糖的培養液飼料，試驗組魚隻之最終平均體重及增重率稍高於對照組，與對照組比較，在統計上無顯著差異 (p > 0.05)，試驗組與對照組魚隻之飼料轉換率 (Feed conversion ratio, FCR) 在統計上亦無顯著差異 (p > 0.05)。消化酵素活性方面，試驗組魚隻腸道中蛋白酶及脂肪酶之活性顯著高於對照組 (p < 0.05)，而澱粉酶活性與對照組無顯著差異 (p > 0.05)。試驗組魚隻腸道中弧菌數均明顯較對照組為低。以上結果顯示，益生菌B. licheniformis FRI MY-55及其所產乳果糖與果聚糖有增加石斑魚消化酵素活性之趨勢 。

The sensitivity of the probiotic Bacillus licheniformis FRI MY-55 to low pH levels and high bile concentrations was tested, as were the effects of the probiotic and its lactosucrose and levan products on the growth performance, digestive enzymes, and viable bacterial counts in the intestines of orange-spotted grouper (Epinephelus coioides). Bacillus licheniformis FRI MY-55 was able to survive in pH 2.5 and 1.0% bile salt. Orange-spotted grouper specimens were fed (1) a control diet, (2) a diet supplemented with B. licheniformis FRI MY-55 (107 CFU/g) + lactosucrose (0.15%) culture medium, or (3) a diet supplemented with B. licheniformis FRI MY-55 (107 CFU/g) + levan (0.15%) culture medium for up to 10 weeks. The final weights and percent weight gains of the spotted grouper specimens fed the diet supplemented with B. licheniformis FRI MY-55 + lactosucrose culture medium and those of the specimens fed the diet supplemented with B. licheniformis FRI MY-55 + levan culture medium were higher than those of the control group specimens, but the differences with the control group were not statistically significant (p> 0.05). There were also no statistically significant differences in the feed conversion rate (FCR) between the experimental groups and the control group (p> 0.05). However, the protease and lipase activities in the digestive tracts of the fish fed the diet supplemented with B. licheniformis FRI MY-55 + lactosucrose culture medium and those fed the diet supplemented with B. licheniformis FRI MY-55 + levan culture medium were significantly increased over those in the digestive tracts of the control group fish (p< 0.05). In contrast, the amylase activities in the digestive tracts of the experimental feed groups were not significantly increased over those in the digestive tracts of the control group fish (p> 0.05). The Vibrio spp. counts in the intestines of the groupers in both experimental groups were significantly decreased compared to those in the digestive tracts of the control group fish. Overall, the results of this study indicate that dietary B. licheniformis FRI MY-55 and its lactosucrose and levan products could provide an effective means for enhancing the activities of the digestive enzymes of orange-spotted grouper.