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海水吳郭魚養殖池中果聚醣生產菌之篩選

  • 出版日期:100-12-30
  • 標題title(英):
    Screening of Levan-producing Bacteria from Brackish Water Tilapia Culture Pond
  • 作者:黃美瑩‧陳柏璇‧黃詩涵‧林金榮‧潘崇良
  • 作者auther(英):Mei-Ying Huang, Po-Hsuan Chen, Shih-Han Huang, Kin-Jong Lin and Chorng-Liang Pan
  • 卷別:19
  • 期別:2
  • 頁碼:77-91

本研究自海水吳郭魚養殖池中篩選出5株具有合成果聚醣能力之細菌 (M1、M3、M6、M11及 T1),比較其蔗糖之利用率、果聚醣產生量及所產果聚醣之分子量分佈情形。結果顯示,在含有 12% 蔗糖培養液中培養168 h (28℃) 期間,菌株M1、M3及M6於48 h所產果聚醣即達較穩定的濃度 (20.35 ~ 23.01 mg/mL),在該時間點此三菌株之蔗糖利用率為75 ~ 84%。M11菌株則培養至96 h 才產出19.81 mg/mL果聚醣,蔗糖利用率為64%。T1 菌株於培養 24 h 時就產生 12.71 mg/mL 的果聚醣,於培養48 h累積達 29.15 mg/mL,蔗糖利用率為92%,之後果聚醣含量持平 (26.78 ~ 31.73 mg/mL),培養至120 h後逐漸下降。有關所產果聚醣之分子量方面,M1、M3、M6、M11及T1等五菌株所產之主要果聚醣 (以GPC結果判斷) 平均分子量分別為 10.79、10.78、10.31、1.78及14.71 KDa,約為其所產果聚醣的 64.31 ~ 89.30%。各菌株所產出主要果聚醣以酸液熱水解處理後,發現其單糖組成份中均以果糖為主;分析其1H NMR及13C NMR圖譜,顯示與文獻上其他論文報告中所述果聚醣的鍵結資料相當接近,因此推斷該5株菌所產果糖聚合物為β(2-6) 鍵結型態的果聚醣。

摘要abstract(英)


This study was devoted to screening of levan-producing bacteria from brackish water tilapia culture pound. Five strains with levan-producing capability were isolated. To investigate the sucrose utilization of the isolates, the yield amount and average molecular weight of produced levan were examined. In addition, the structural characteristics of the levans were analyzed by partial acid hydrolysis and nuclear magnetic resonance (NMR) spectroscopy. During the 168 h incubation period in the medium containing 12% sucrose at 28℃, strains M1, M3, and M6 produced 20.35-23.01 mg/mL levan after 48 h incubation, while the sucrose in the medium has been used in the range of 75-84%. The amount of levan produced by strain M11 was 19.81 mg/mL after 96 h incubation, and its sucrose utilizations rate only reached 64%. Strain T1 from 24 h to 48 h incubation had produced levan 12.71 mg/mL and 29.15 mg/mL, respectively. After 48 h and till 120 h, the amount of produced levan is keeping evenly to 29 mg/mL, as its sucrose utilization rate was 92%. The amount of levan then decreased gradually with prolonged incubation time after 120 h incubation. The main average molecular weight of levans produced by the isolates M1, M3, M6, M11, and T1 were 10.79, 10.78, 10.31, 1.78 and 14.71 KDa, respectively. The hydrolysates of derived from acid and heating treatments of the levan were mainly fructose. The 1H NMR and 13C NMR spectra of the products of the 5 isolates were very similar to peak positions for levan produced. Based on obtain data, the fructans produced by the 5 isolates were found to be the levan containing β(2-6)-linked backbone.