農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

本研究係評估半葉馬尾藻 (Sargassum hemiphyllum) 酒精萃取物的抗氧化活性及探討萃取物對過氧化氫 (Hydrogen peroxide, H₂O₂) 誘發3T3-L1脂肪細胞氧化損傷之影響。半葉馬尾藻酒精萃取物的總酚含量為22.35±1.41 mg/g 乾藻，其抗氧化活性以還原力、清除α,α-Diphenyl-β-picrylhydrazyl (DPPH) 自由基能力、螯合亞鐵離子能力及Superoxide dismutase-like (SOD-like) 活性四項方法測定，結果顯示：半葉馬尾藻酒精萃取物在清除DPPH自由基能力及SOD-like活性方面具有較好的能力。此外，半葉馬尾藻酒精萃取物可以降低H₂O₂ 對3T3-L1脂肪細胞的氧化損傷，及減少胞內活性氧分子 (Reactive oxygen species, ROS) 的量，進而提升細胞的存活率。在半葉馬尾藻酒精萃取物1.5及2.0 mg/ml濃度下，其可提升3T3-L1氧化損傷細胞中的麩胱甘肽過氧化酶 (Glutathione peroxidase, GPx)、過氧化氫酶 (Catalase, CAT) 與超氧化物歧化酶 (Superoxide dismutase, SOD) 活性，及抑制氧化酵素gp91^phox 及p67^phox的mRNA表現量。從上述結果得知，半葉馬尾藻酒精萃取物可能藉由多酚物質之抗氧化能力先清除胞外自由基，進而減少胞內的ROS產生，進而藉由提升抗氧化酵素活性及抑制氧化酵素mRNA表現量，達到減緩H₂O₂ 對3T3-L1脂肪細胞所造成的氧化損傷。

In this study, the antioxidative and cytoprotective effect of Sargassum hemiphyllum ethanolic extract against hydrogen peroxide (H₂O₂)-stimulated 3T3-L1 adipocytes was investigated. The amount of total phenolic compounds in S. hemiphyllum ethanolic extract was 22.35 ± 1.41 mg/g dry seaweed. The antioxidative activity of S. hemiphyllum ethanolic extract was evaluated using four different methods, including reducing power, DPPH free radicals scavenging activity, Fe₊₂ chelating activity and SOD-like activity. The results showed that S. hemiphyllum ethanolic extract had the potential DPPH free radicals scavenging and SOD-like activities. The S. hemiphyllum ethanolic extract significantly reduced the intracellular reactive oxygen species (ROS) that produced by H₂O₂ stimulating, and it also raised cell survivability. At 1.5 and 2.0 mg/ml, S. hemiphyllum ethanolic extract increased the activities of antioxidant enzymes such as superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT), and decreased its mRNA expression of gp91^phox and p67^phox of NADPH oxidase subunits. Thus, it is suggested that S. hemiphyllum ethanolic extract increasingly protected 3T3-L1 adipocytes against oxidative damage by the cellular antioxidant activity and inhibited oxidase mRNA expression.