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牙鮃的誘導產卵及幼苗發育

  • 出版日期:106-12-31
  • 標題title(英):
    Induced Spawning and Larval Rearing of Olive Flounder (Paralichthys olivaceus)
  • 作者:陳玉萍•陳鏗元•劉恩良•何源興
  • 作者auther(英):Yu-Ping Chen, Keng-Yuan Chen, En-Lieng Lau and Yuan-Shing Ho
  • 卷別:25
  • 期別:2
  • 頁碼:43-53

本研究利用溫差刺激順利誘導牙鮃 (Paralichthys olivaceus) 種魚於人工飼養環境下自行產卵,其受精率為81.2%。受精卵為圓形透明之浮性卵,平均卵徑為0.94 ± 0.01 mm,油球徑為0.16 ± 0.01 mm。在水溫22 ± 1℃下受精卵約32小時孵化,剛孵化之魚苗平均全長為2.12 ± 0.03 mm。第3日齡仔魚平均全長為3.47 ± 0.76 mm,開始投餵輪蟲,並添加微藻以穩定水質及滋養輪蟲。第6日齡平均全長為4.58 ± 0.18 mm,此時可混投豐年蝦,豐年蝦需經營養強化。第18日齡平均全長為5.82 ± 0.21 mm,頭部冠狀幼鰭開始生長,持續投餵營養強化之豐年蝦。第24日齡平均全長為8.38 ± 0.11 mm,此時游泳姿勢傾斜,眼睛相對位置開始改變。第38日齡平均全長為13.88 ± 1.26 mm,眼睛位於同一側完成變態階段,開始進入底棲生活。將海水鹽度設定在5–40 psu之間,在不同鹽度下,各實驗組受精卵之孵化率有顯著差異 (p < 0.05),以鹽度30 psu組的孵化率77.8 ± 1.4% 較佳。仔魚在孵化後72 hr,20及30 psu實驗組有較佳活存率,分別為72% 與75%。

摘要abstract(英)


This study successfully induced spawning and fertilization of the olive flounder (Paralichthys olivaceus) in an artificial feeding environment using temperature stimulation, achieving a fertilization rate of 81.2%. The fertilized eggs of the fish were buoyant and semi-transparent, with an average diameter of 0.94 ± 0.01mm and an oil globule averaging 0.16 ± 0.01 mm in diameter. These eggs hatched at 32 hr after fertilization (Temperature = 22 ± 1℃; salinity = 33–35 psu). The newly hatched larvae averaged about 2.12 ± 0.03 mm in total length. The larvae (3.47 ± 0.76 mm) were fed with rotifers (Brachionus plicatilis) from the 3rd day post hatching (DPH). A microalgae (Nannochloropsis oculata) was added to stabilize the water quality and nourish the rotifers. The average total length of the 6 DPH fry was 4.58 ± 0.18 mm. Artemia (Artemia spp.) were used as a feed additive for nutrition enrichment. The average total length of the 18 DPH fry was 5.82 ± 0.21 mm. Then, the coronary fin on the head of each larva began to grow. At this time, we continued to use the artemia for nutrition enrichment. The average total length of the 24 DPH fry was 8.38 ± 0.11 mm; their swimming was tilted and the relative position of their eyes began to change. When the eyes were on the same side at 38 DPH (13.88 ± 1.26 mm), the fry began to metamorphose to the benthic stage. The salinity level was then set to 5–40 psu. In the salinity test, it was found that a significantly higher hatching rate (77.8 ± 1.4%) was obtained at 30 psu (p < 0.05). Furthermore, through salinity tests with different incubation times (24, 48, or 72 hr), this study found that the 20 psu and 30 psu tests had an optimum survival rate of 72% and 75%, respectively, at 72 hr of treatment.