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馬尾藻生理活性物質及其應用於豹鱠養殖之研究

  • 出版日期:106-12-31
  • 標題title(英):
    The Physiological Activity of Sargassum spp. Extracts in Aquatic Feed for Plectropomus leopardus
  • 作者:林慧秋‧許鐘鋼‧廖紫嬿‧高雪卿‧林金榮
  • 作者auther(英): Hui-Chiu Lin, Chung-Kang Hsu, Tzu-Yen Liao, Hsueh-Ching Kao and Kim-Jung Lin
  • 卷別:25
  • 期別:2
  • 頁碼:55-68

本試驗為開發馬尾藻酵素水解萃取物粉末添加於飼料中用以餵養豹鱠並評估抗菌、抗氧化、酵素活性等效益。結果顯示,馬尾藻酵素水解物具有極佳的DPPH及ABTS自由基清除活性;對於仙人掌桿菌、金黃色葡萄球菌、大腸桿菌、表皮葡萄球菌、枯草桿菌,具有良好的抗菌及抑菌功能。以1 - 4% 馬尾藻粉末添加於豹鱠飼料中來餵養,結果顯示豹鱠之成長及魚肉一般成分,各組間幾乎沒有差異,但添加馬尾藻有效優化豹鱠之腸道微生物菌相。另外,豹鱠血液免疫指數-血清蛋白比值以馬尾藻酵素水解粉末添加1、2及4% 較佳,抗蛋白酶活性以3及2% 添加較佳,溶菌酶以4及2% 添加較佳,呼吸爆則以1 - 3% 添加較佳。而豹鱠以馬尾藻酵素水解粉末添加飼養8週後,其魚肉一般成分除了粗脂肪以2及4% 較佳外,其他皆無差異。綜合以上結果顯示,以馬尾藻酵素水解粉末添加2及4% 佳,但基於成本考量及其效益,建議以添加最適2%。未來,海藻可應用於水產養殖之潛力值得關注。

摘要abstract(英)


The purpose of this study was to develop Sargassum enzyme hydrolysis extract powders to be added to the diets of Plectropomus leopardus and to assess their effects in terms of antibacterial, antoxidant, and enzyme activity. The results indicated that the Sargassum enzyme hydrolysis extracts had good antibacterial and bacteria-inhibiting effects against Bacillus cereus, Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, and Vibrio parahaemolyticus, as well as high DPPH and ABTS radical scavenging activity. The growth and proximate analysis results of P. leopardus fed with diets supplemented with Sargassum enzyme hydrolysis extracts were not significantly different from those of fish fed with a control diet. However, P. leopardus fed with diets supplemented with Sargassum enzyme hydrolysis extracts had optimum intestinal microbial flora. According to a blood immune analysis of P. leopardus, the serum protein ratio was better in fish fed with the addition of 1%, 2%, and 4% of the hydrolyzed powder of Sargassum. The antiprotease activity was better at 3% and 2% added, and the lysozyme activity was better with 4% and 2% added. In terms of the proximate composition of muscle of P. leopardus meat after being fed with Sargassum feed formulations for 8 weeks, the level of crude fat was better when the Sargassum composition of the diet was 2% or 4%, while there were no significant differences at other composition levels. According to the above findings, better results were achieved when the hydrolyzed Sargassum powder composition of the diet was 2% or 4%, but a composition of 2% appeared to be best when the cost and benefits were considered. More generally, the results indicate that diets supplemented with seaweed in aquaculture.