In order to select a good breed both with ideal culture performance and high salinity tolerance, taking Taiwanese red tilapia TsRn line Oreochromis spp and TFS line Oreochromis Spilurus as control groups, two reciprocal hybrids, TsRn♀×TFS♂ and TFS♀×TsRn♂ as trial groups. The major results were: (1) TsRn♀×TFS♂, this mating can produce fertilized egg but couldn’t hatch out. (2) F1 breeding from TFS♀×TsRn♂ can adapt to show a better salinity tolerance, as the same Oreochromis Spilurus. Comparison were conducted under 4 salinities (5, 15, 15, 30) acclimating progressively to rise 10 salinity from salinities 5 to sea increasing every week, after swimming fry for high salinity tolerance study . In the period of acclimated salinity, the survival rates of TsRn F3 was 36.21 % (174/480), 86.47 % (805/931) in TFS, and 83.19 % (952/1145) in F1 produced from TFS♀×TsRn♂. All survival fishes after acclimated salinity were reared to adult for 3 month, comparison to survival rates of salinity tolerance between TsRn, TFS and F1 produced from TFS♀×TsRn♂, the survival rate of salinity tolerance was 74.03 % (129/174) in TsRn, 93.77 % (755/805) in TFS and 92.28 % (878/952) in F1 produced from TFS♀×TsRn♂. This suggests that the traits of survival rate of salinity tolerance were significantly raised by the Oreochromis Spilurus.