In this research project, TFS♂ ×TsRn♂ has produced F1 and F2 offspring. Among them, F2 can be divided into three groups based on phenotype: black group, black dots with red background, and black spots with red bottom. In addition, only the black group F2 can produce F3 generation, and the red group with black spots and red spots with black spots cannot produce offspring. F1 (produced by TFS♀ ×TsRn♂) and TsRn can not breed offspring, but F1 and TFS can produce offspring by backcrossing. The offspring of TsRn♀ ×TFS♂ cannot survive effectively due to seawater adaptation problems. Therefore, this year use TsRn♀, which go through several years of progressive acclimation, paired with TFS for the first time produce offspring, which survival and body color and body are better than TFS♂ ×TsRn♂ offspring. Therefore, we result out the seawater adaptation traits come from TFS, body color and body traits come from the female parent.