1. In order to understand practicablity cryopreserved sperm of small abalone (Haliotis diversicolor )sperm, which have established in 2016, using this sperm that has been cryopreserved in liquid nitrogen (LN) for 180 days under the same thawing conditions fertilized test at concentration of 102 / mL to 103 / mL at 23-25 ° C, comparing the deformity of embryo stage and the control group, there were respectively 46% and 28%, and the hatch-out was respectively 67 (0.00015%) and 114 (0.00026%). The results showed that the length of time for cryopreservation in LN has a great impact on subsequent development. 2. In study the cryopreservation of Epinephelus coioides and Oplegnathus fasciatus sperm , mature E. coioides semen and O. fasciatus semen were used as experimental material, and concentration of various semen diluents, and concentration of cryoprotectant were screened.We used liquid nitrogen and 0.5 ml cryo-straws to preserve sperms by step cooling procedure. The result showed the cryopreservation of E. coioides semen that Hank’s solution、Marine Fish Ringer and EM1-2 semen duluents was better , which preserved 73.00%±2.00、71.67%±1.53及68.00%±3.00 sperm motility after unfrozen. Using Hank’s solution as the liquid foundation to prepare cryoprotectant, no significant difference was observed in sperm motility by 12% and 18% DMSO or Propylene Glycol cryopreservation (P>0.05) . The best of all is 12% DMSO, which preserved 91.00% ± 2.00 sperm motility respectively after unfrozen. The cryopreservation of O. fasciatus semen that Hank’s solution semen diluents was better in sperm motility, which preserved 54.33% ± 7.37% sperm motility after unfrozen. Using Hank’s solution as the liquid foundation to prepare cryoprotectant, no significant difference was observed in sperm motility by 12% Glycerin cryopreservation, which preserved 85.67% ±4.51 sperm motility respectively after unfrozen.Using cryopreserved E.lanceolatus semen to fertilize E. coioides eggs, the rates of fertilization were 97.72%±1.64 and 96.72%±1.09 without difference with fresh sperms, the rates of hatching were 79.22%±2.88 and 78.27%±2.21. We established a sperm cryopreservation protocol for E. coioides and O. fasciatus on this study, and it will provide a basis for the artificial breeding and cross breeding. 3. The purpose of this program is to provide the tilapia industry with new techniques that were developed by the Germplasm Bank of Freshwater Fish, Fisheries Research Institute. These techniques will be promoted to the industry with following goals to enhance the competitive strength.The technology transfer of genetically male tilapia.The supply of 500,000 fries of Nile tilapia with the feature of high growth rate.The application of molecular protocols to analysis the reason of low maleratio of tilapia fry.Although the FRI could provide the aquaculture industry with a high-growth tilapia to commercialization but could not match and through licensing agreements in this year. In addition, we try to analyze the sex-determining markers of genetically both female and male ,try to realize the growth characters. The results show that the final weight in the group C (genetic female and male) with group D (genetic male only) have no significant difference. Otherwise, the genetic female marker could be applied on genetic breeding in the future. 4.Symbiotic alga of Tridacna maxima were isolated and cultivated from artificially produced juveniles with different mantle colorations. Molecular genetic data verified all symbiotic alga from different hosts are the same clade (A). It means different mantle colorations of T. maxima are not determined by symbiotic alga. The cultivated alga were also prepared for infection trail for artificially produced larvae. Byssus of juveniles with shell length of 1 cm will regenerate within 24 hours whereas those of 3-6 cm will do in 5-7 days. One-year survival rates of transplanted juveniles in 2017 are between 0-20%, while those protected in cage are 96.3%.